Departamento de Biologia Geral, Centro de Ciências Biológias, Campus Universitário, Universidade Estadual de Londrina, Cx. Postal 6001, Londrina, Paraná, CEP 86051-990, Brasil.
Environ Toxicol Pharmacol. 2005 Feb;19(2):197-201. doi: 10.1016/j.etap.2004.03.011.
Testing for environmental pollutants is an ever-growing concern. Various tests in organisms have been utilized for the detection and identification of toxic substances in the air, water and soil. In the present study, we utilized the comet assay in Tilapia rendalli to conduct an environmental assessment of Lake Igapó II, a lake located in the metropolitan area of Londrina, PR-Brazil. The results demonstrated that samples from Lake Igapó II had a significantly greater number of comets, mainly in classes 2 and 3. The results suggest a genotoxicity of the aquatic environment at Lake Igapó II and that the comet assay in T. rendalli provides adequate sensitivity to be utilized as a tool in the monitoring of water pollution and environmental risk assessment.
环境污染物检测是一个日益受到关注的问题。目前,已经在各种生物体中利用各种检测方法来检测和识别空气中、水中和土壤中的有毒物质。在本研究中,我们利用丽鱼(Tilapia rendalli)的彗星试验对位于巴西 PR-朗多尼亚州洛里纳市大都市区的伊加波 II 湖进行了环境评估。结果表明,伊加波 II 湖的样本中彗星数量明显更多,主要集中在 2 类和 3 类。这些结果表明伊加波 II 湖的水生环境具有遗传毒性,丽鱼的彗星试验具有足够的灵敏度,可以作为监测水污染和环境风险评估的工具。