新型蛋白靶标用于大鼠脑内有机磷农药。

Novel protein targets for organophosphorus pesticides in rat brain.

机构信息

MRC Applied Neuroscience Group, School of Biomedical Sciences, University of Nottingham, Queens Medical Centre, Nottingham NG7 2UH, UK.

出版信息

Environ Toxicol Pharmacol. 2005 May;19(3):451-4. doi: 10.1016/j.etap.2004.12.006. Epub 2005 Jan 26.

DOI:10.1016/j.etap.2004.12.006

PMID:21783511

Abstract

We report preliminary results from a proteomic search for rat brain protein targets adducted by organophosphorous pesticides. Azamethaphos, chlorfenvinphos, diazinon, malathion and chlorpyrifos oxons (in rat brain homogenates) or pirimiphos-methyl (after systemic treatment) were tested at levels producing no more than 30% inhibition of brain acetylcholinesterase. Loss of reactivity with tritiated diisopropylflurophosphate was taken as proof of adduction by the test organophosphate. In addition to acetylcholinesterase other, previously unrecognised, adducted proteins were detected in total brain protein extracts at 30, 32, 41, 71 and 83kDa. Azamethiphos adducted all but the 30 and 32kDa bands, but chlorpyrifos only acetylcholinesterase.

摘要

我们报告了一项蛋白质组学研究的初步结果，该研究旨在寻找有机磷杀虫剂在大鼠脑中的靶蛋白加合物。在产生不超过 30%的脑乙酰胆碱酯酶抑制作用的水平下，检测了唑磷、氯芬磷、二嗪磷、马拉硫磷和毒死蜱氧（在大鼠脑匀浆中）或吡虫啉甲基（在系统治疗后）。与氚化二异丙基氟磷酸酯反应性丧失被视为测试有机磷化合物加合的证据。除乙酰胆碱酯酶外，在全脑蛋白提取物中还检测到其他以前未识别的加合蛋白，分子量分别为 30、32、41、71 和 83kDa。唑磷除了 30 和 32kDa 条带外，均可与所有蛋白结合，但毒死蜱仅与乙酰胆碱酯酶结合。