Department of Public Health, University of Szeged, Dóm tér 10, H-6720 Szeged, Hungary.
Environ Toxicol Pharmacol. 2005 May;19(3):797-810. doi: 10.1016/j.etap.2004.12.046.
In male Wistar rats, behavioral and electrophysiological investigations, and blood and brain manganese level determinations, were performed; during 10 weeks treatment with low-dose manganese chloride and a 12 weeks post-treatment period. Three groups of 16 animals each received daily doses of 14.84 and 59.36mg/kg b.w. MnCl(2) (control: distilled water) via gavage. During treatment period, Mn accumulation was seen first in the blood, then in the brain samples of the high-dose animals. Short- and long-term spatial memory performance of the treated animals decreased, spontaneous open field activity (OF) was reduced. The number of acoustic startle responses (ASR), and the pre-pulse inhibition (PPI) of these, diminished. In the cortical and hippocampal spontaneous activity, power spectrum was shifted to higher frequencies. The latency of the sensory evoked potentials increased, and their duration, decreased. By the end of the post-treatment period, Mn levels returned to the control in all samples. The impairment of long-term spatial memory remained, as did the number of acoustic startle responses. Pre-pulse inhibition, however, returned to the pre-treatment levels. The changes of the open field activity disappeared but a residual effect could be revealed by administration of d-amphetamine. The electrophysiological effects were partially reversed. By applying a complex set of methods, it was possible to obtain new data for a better-based relationship between the known effects of Mn at neuronal level and the behavioral and electrophysiological outcomes of Mn exposure.
在雄性 Wistar 大鼠中进行了行为和电生理研究，并测定了血液和大脑中的锰水平；在为期 10 周的低剂量氯化锰治疗和 12 周的治疗后期间进行了这些研究。三组 16 只动物，每组每天通过灌胃接受 14.84 和 59.36mg/kg b.w. MnCl(2)（对照：蒸馏水）。在治疗期间，高剂量动物的血液和大脑样本中首先观察到 Mn 积累。受处理动物的短期和长期空间记忆表现下降，自发开放场活动（OF）减少。声发射反应（ASR）的数量减少，其预脉冲抑制（PPI）减少。在皮质和海马体的自发活动中，功率谱移向更高的频率。感觉诱发电位的潜伏期增加，其持续时间减少。在治疗后期间结束时，所有样本中的 Mn 水平均恢复到对照水平。长期空间记忆的损害仍然存在，声发射反应的数量也是如此。然而，预脉冲抑制恢复到治疗前的水平。开放场活动的变化消失了，但通过给予 d-苯丙胺仍可揭示残留效应。电生理效应部分逆转。通过应用一系列复杂的方法，有可能获得新的数据，以便更好地建立 Mn 在神经元水平上的已知作用与 Mn 暴露的行为和电生理结果之间的关系。
