Immunology and Reproductive Biology Laboratory, Medical School, Nanjing University, Nanjing, Jiangsu 210093, PR China.
Environ Toxicol Pharmacol. 2005 Jul;20(1):182-7. doi: 10.1016/j.etap.2004.12.060.
Pentachlorophenol (PCP) is the most toxic contaminant of chlorophenols (CPs). Due to improper disposal, PCP has become an environmental pollutant and is now considered to be ubiquitous. Previous studies about the influences of PCP on reproductive function were mostly focused on experiments in vivo. The aim of our present study was to estimate the toxic effects of PCP on cultured Sertoli cells from Sprague-Dawley rats. The viability of Sertoli cells was detected and morphological examination was performed followed by flow cytometric assay to evaluate its toxic effects. The 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide (MTT) assay showed that the toxic effects of PCP on cultured Sertoli cells were dose- and time-dependent. By flow cytometric assay, it was found that the number of dead Sertoli cells increased with the increase of exposed PCP levels. The results indicated that PCP had direct and dose-dependent cytotoxic effects on Sertoli cells in vitro.
五氯苯酚（PCP）是氯苯酚（CPs）中毒性最大的污染物。由于处理不当，PCP 已成为一种环境污染物，现在被认为是无处不在的。以前关于 PCP 对生殖功能影响的研究大多集中在体内实验上。本研究旨在评估 PCP 对 Sprague-Dawley 大鼠培养的支持细胞的毒性作用。检测支持细胞的活力，进行形态学检查，然后进行流式细胞术分析，以评估其毒性作用。3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）检测结果表明，PCP 对培养的支持细胞的毒性作用具有剂量和时间依赖性。通过流式细胞术分析，发现随着暴露于 PCP 水平的增加，死亡的支持细胞数量增加。结果表明，PCP 对体外支持细胞具有直接的、剂量依赖性的细胞毒性作用。
