Holsapple M P, McNerney P J, McCay J A
J Toxicol Environ Health. 1987;20(3):229-39. doi: 10.1080/15287398709530977.
The effects of pentachlorophenol (PCP) on selected parameters reflecting immunocompetence of female B6C3F1 mice were measured following subchronic exposure (14 d) and direct exposure in culture. Daily exposure was by gastric intubation of 10, 30, or 100 mg/kg of technical-grade PCP (PCP-T), or 100 mg/kg of EC-7, a PCP preparation purified to reduce contamination (PCP-P), or 100 mg/kg of the vehicle, corn oil. There were no effects on the antibody responses of spleen-cell suspensions from either PCP-T- or PCP-P-treated mice stimulated with antigen in culture. In contrast, when mice were immunized during the exposure to PCP-T, there was a dose-related suppression of the IgM antibody response to sheep red blood cells (SRBC) measured on both d 4 (peak day) and d 5. There was no change in the antibody response of mice exposed to PCP-P. The differential activity was not observed following direct addition, since both PCP-T and PCP-P suppressed the in vitro antibody response by spleen-cell suspensions from untreated mice. The suppression was associated with a decrease in cell viability, indicating that both preparations were directly cytotoxic. These results indicate that the in vitro antibody assay will be of limited value in determining the mechanism of immunosuppression by PCP. The lack of effect on the antibody response by splenocytes from PCP-T-treated mice indicates that the dysfunction is not due to a direct suppression of the capabilities of immunocompetent cells.
在亚慢性暴露（14天）及培养物直接暴露后，测定了五氯苯酚（PCP）对反映雌性B6C3F1小鼠免疫能力的选定参数的影响。每日通过胃插管给予10、30或100mg/kg的工业级PCP（PCP-T），或100mg/kg的EC-7（一种为减少污染而纯化的PCP制剂，PCP-P），或100mg/kg的赋形剂玉米油。在用抗原刺激培养的情况下，PCP-T或PCP-P处理的小鼠脾脏细胞悬液的抗体反应均未受影响。相比之下，当小鼠在暴露于PCP-T期间进行免疫时，在第4天（峰值日）和第5天测量的对绵羊红细胞（SRBC）的IgM抗体反应出现了剂量相关的抑制。暴露于PCP-P的小鼠的抗体反应没有变化。直接添加后未观察到差异活性，因为PCP-T和PCP-P均抑制了未处理小鼠脾脏细胞悬液的体外抗体反应。这种抑制与细胞活力下降有关，表明两种制剂均具有直接细胞毒性。这些结果表明，体外抗体测定在确定PCP免疫抑制机制方面的价值有限。PCP-T处理的小鼠脾细胞对抗体反应缺乏影响，表明功能障碍并非由于免疫活性细胞能力的直接抑制所致。
