Biosafety Research Institute, Department of Pathology, College of Veterinary Medicine, Chonbuk National University, Jeonju, 561-756, South Korea.
Environ Toxicol Pharmacol. 2006 Jan;21(1):22-33. doi: 10.1016/j.etap.2005.05.011. Epub 2005 Jul 20.
Angiogenesis is a vital process in the growth of new blood vessels from pre-existing vasculature. Among several approaches being used for studies related to angiogenesis, chicken chorioallantoic membrane assay (CAM) is an excellent model system. However, its utility has been limited due to difficulty in quantifying putative angiogenic and anti-angiogenic response to an experimental compound in an objective and quantifiable manner. Herein, we report a novel approach of image acquisition and processing for better evaluation of neovascularization. The effects of mainstream cigarette smoke solutions (MSCSS) and sidestream cigarette smoke solutions (SSCSS) from different commercially available cigarettes on angiogenesis were quantified, using CAM assay. Different gross and nanometer scale topographies of CAMs were quantified, which are vital for 3D image scrutiny and can precisely enumerate angiogenesis. Pattern formation of blood vessels, diameter, area and 3D surface roughness of CAMs were substantially disrupted by application of cigarette-smoke extracts. An important point revealed in our study that SSCSS appeared to be significantly more toxic than MSCSS with respect to their effects on angiogenesis. This new imaging technique combined with other modalities, will provide a robust platform to optimize trial design and more patent studies in angiogenesis.
血管生成是新血管从现有脉管系统生长的关键过程。在用于血管生成相关研究的几种方法中，鸡胚绒毛尿囊膜（CAM）分析是一种出色的模型系统。然而，由于难以客观和定量地量化实验化合物对新血管生成和抗血管生成的反应，其应用受到限制。在此，我们报告了一种新的图像采集和处理方法，以更好地评估新血管生成。使用 CAM 分析，定量检测了来自不同市售香烟的主流香烟烟雾溶液（MSCSS）和侧流香烟烟雾溶液（SSCSS）对血管生成的影响。定量了 CAM 的不同总貌和纳米级形貌，这对于 3D 图像检查至关重要，并可以精确计数新血管生成。香烟烟雾提取物的应用显著破坏了血管的图案形成、直径、面积和 CAM 的 3D 表面粗糙度。我们的研究揭示了一个重要观点，即与 MSCSS 相比，SSCSS 对血管生成的影响似乎毒性更大。这种新的成像技术与其他模式相结合，将为优化试验设计和更多血管生成专利研究提供一个强大的平台。
