Environmental Toxico-Genomic and Proteomic Center, College of Medicine, Korea University, 5 Anamdong Sungbukku, Seoul 136-701, South Korea.
Environ Toxicol Pharmacol. 2006 Jan;21(1):108-17. doi: 10.1016/j.etap.2005.08.004. Epub 2005 Oct 5.
In this study, we investigated the immunotoxicities of polycyclic aromatic hydrocarbons in 54 automobile emission inspectors and in 84 control subjects, and evaluated associations between immunological and genotoxicological parameters. Specific surface antigens of peripheral lymphocytes, namely, CD3, CD4, CD8, CD19, and CD69 were subjected to measure immune status in automobile emission inspectors and control subjects. T-and B-cells showed no significant differences between automobile emission inspectors and control subjects (p=0.740 and 0.395). In addition, the ratio of T helper cells to T cytotoxic cells was not deferent (p=0.144). However, T-cell activation was found to be significantly higher in automobile emission inspectors (p=0.041), but not B-cell activation. The levels of two cytokines (IL-4 an INF-γ) and four immunoglobulins (IgA, IgE, IgG, and IgM) were also determined in automobile emission inspectors and control subjects. All immunoglobulin types were lower in automobile emission inspectors, but this was significant only for IgG (0.047). In addition, the levels of two cytokines, IL-4 and INF-γ, were also higher in automobile emission inspectors, though this was not significant. DNA damage in mononuclear and polynuclear lymphocytes and in the level of urinary metabolites, 1-OHP and 2-naphthol, were evaluated in automobile emission inspectors and in control subjects and significant differences were found between the two groups. Examinations of urinary metabolites, DNA damage, and immunological parameters, including leukocyte subpopulations, immunoglobulins, and cytokines, showed that the cytokines levels were associated with the levels of two urinary metabolites, 1-OHP and 2-naphthol.
在这项研究中，我们调查了 54 名汽车排放检验员和 84 名对照者体内多环芳烃的免疫毒性，并评估了免疫和遗传毒性参数之间的相关性。外周淋巴细胞的特异性表面抗原，即 CD3、CD4、CD8、CD19 和 CD69，用于测量汽车排放检验员和对照者的免疫状态。T 细胞和 B 细胞在汽车排放检验员和对照者之间没有显著差异（p=0.740 和 0.395）。此外，辅助性 T 细胞与细胞毒性 T 细胞的比例没有差异（p=0.144）。然而，T 细胞的激活在汽车排放检验员中明显更高（p=0.041），而 B 细胞的激活则不然。在汽车排放检验员和对照者中还测定了两种细胞因子（IL-4 和 INF-γ）和四种免疫球蛋白（IgA、IgE、IgG 和 IgM）的水平。所有免疫球蛋白类型在汽车排放检验员中均较低，但仅 IgG 具有统计学意义（0.047）。此外，两种细胞因子（IL-4 和 INF-γ）的水平在汽车排放检验员中也较高，尽管这没有统计学意义。在汽车排放检验员和对照者中评估了单核和多核淋巴细胞的 DNA 损伤以及尿代谢物 1-OHP 和 2-萘酚的水平，发现两组之间存在显著差异。对尿代谢物、DNA 损伤以及包括白细胞亚群、免疫球蛋白和细胞因子在内的免疫参数的检查表明，细胞因子水平与两种尿代谢物 1-OHP 和 2-萘酚的水平有关。
