University of Çukurova, Faculty of Science and Letters, Department of Biology, 01330 Balcalı, Adana, Turkey.
Environ Toxicol Pharmacol. 2006 May;21(3):241-5. doi: 10.1016/j.etap.2005.08.007. Epub 2005 Oct 26.
The aim of this study was to investigate the effects of organophosphorus (OP) pesticide diazinon on acetylcholinesterase (AChE: EC 3.1.1.7) activity and its relationship to lipid peroxidation (LPO) in the brain of a freshwater fish, Oreochromis niloticus. Malondialdehyde (MDA) content was used as biomarker for LPO. Fish were exposed to 1 and 2mg/L sublethal concentrations of diazinon for 1, 7, 15 and 30 days. In the entire experimental group, AChE activity in brain significantly decreased (up to 93% of control), whereas MDA content decreased after 1 day, and increased after 7 and 15 days of exposures. MDA was in similar level with the control group after diazinon exposure of 30 days. The findings of the present study show that diazinon inhibited AChE activity and it has LPO-inducing potential in fish. The inhibition of AChE activity in the brain of O. niloticus correlated with increased MDA levels after 7 and 15 days diazinon exposures (r=-0.661, P<0.019; r=-0.652, P<0.022, respectively).
本研究旨在探讨有机磷（OP）农药敌百虫对淡水鱼类奥利亚罗非鱼脑乙酰胆碱酯酶（AChE：EC 3.1.1.7）活性的影响及其与脂质过氧化（LPO）的关系。丙二醛（MDA）含量用作 LPO 的生物标志物。鱼被暴露于 1 和 2mg/L 的敌百虫亚致死浓度下 1、7、15 和 30 天。在整个实验组中，脑 AChE 活性显著降低（达对照组的 93%），而 MDA 含量在暴露 1 天后降低，在暴露 7 和 15 天后增加。敌百虫暴露 30 天后，MDA 与对照组水平相似。本研究的结果表明，敌百虫抑制了鱼类的 AChE 活性，并有诱导 LPO 的潜力。奥利亚罗非鱼脑 AChE 活性的抑制与暴露于敌百虫 7 和 15 天后 MDA 水平的升高相关（r=-0.661，P<0.019；r=-0.652，P<0.022）。
