Departamento de Química e Ciências Ambientais, IBILCE / UNESP, R Cristóvão Colombo 2265, CEP 15054-000, São José do Rio Preto, SP, Brazil.
Ecotoxicol Environ Saf. 2010 Jul;73(5):858-63. doi: 10.1016/j.ecoenv.2010.01.016. Epub 2010 Feb 9.
Biochemical biomarkers (the activities of acetylcholinesterase, 7-ethoxyresorufin-O-deetilase, carboxylesterase, catalase, glutathione peroxidase and glutathione S-transferase) were evaluated in Nile tilapia (Oreochromis niloticus) that had been exposed to benzo[a]pyrene (BaP) and the organophosphate pesticide diazinon (DZ), at 0.5mg/L. The animals were pre-exposed to BaP for three days, and DZ was then added to both non-exposed and pre-exposed groups, being exposed for 2 and 7 additional days. The level of BaP was also measured in the bile. BaP caused the induction of phase I and II enzymes, and DZ caused carboxylesterase inhibition in gills but not in liver. AChE activity was unchanged. No significant modulation was observed in antioxidant enzymes. When in combination with BaP, DZ caused a significant decrease of EROD and GST induction. Levels of BaP in the bile were also increased in fish exposed to BaP combined with DZ, indicating an interference of DZ in responses activated by BaP.
生化标志物（乙酰胆碱酯酶、7-乙氧基试卤灵-O-去乙基酶、羧酸酯酶、过氧化氢酶、谷胱甘肽过氧化物酶和谷胱甘肽 S-转移酶的活性）在尼罗罗非鱼（Oreochromis niloticus）中进行了评估，这些鱼曾暴露于苯并[a]芘（BaP）和有机磷农药二嗪农（DZ）中，浓度为 0.5mg/L。动物先暴露于 BaP 中三天，然后将 DZ 添加到非暴露组和预暴露组中，再分别暴露两天和七天。还测量了胆汁中的 BaP 水平。BaP 诱导了 I 期和 II 期酶，DZ 抑制了鳃中的羧酸酯酶，但不抑制肝脏中的羧酸酯酶。AChE 活性未发生变化。抗氧化酶未见明显调节。当与 BaP 联合使用时，DZ 导致 EROD 和 GST 诱导显著降低。与 DZ 联合暴露于 BaP 的鱼的胆汁中的 BaP 水平也增加，表明 DZ 干扰了 BaP 激活的反应。
