Srinivasan M, Sudheer A Ram, Pillai K Raveendran, Kumar P Raghu, Sudhakaran P R, Menon V P
Department of Biochemistry, Faculty of Science, Annamalai University, Annamalainagar 608002, Tamil Nadu, India.
Environ Toxicol Pharmacol. 2007 Sep;24(2):98-105. doi: 10.1016/j.etap.2007.03.001. Epub 2007 Apr 6.
Ionizing radiation is known to induce oxidative stress through generation of reactive oxygen species (ROS) resulting in imbalance of the pro-oxidant and antioxidant in the cells, which is suggested to culminate in cell death. The present work was aimed to evaluate the radioprotective effect of curcumin, a yellow pigment of turmeric on γ-radiation-induced toxicity in primary cultures of isolated rat hepatocytes. Hepatocytes were isolated from the liver of rats by collagenase perfusion. The cellular changes were estimated using lipid peroxidative indices like thiobarbituric acid reactive substances (TBARS), the antioxidants superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and reduced glutathione (GSH), ceruloplasmin, vitamins A, E and C and uric acid. The comet assay is a sensitive and rapid technique for quantifying and analyzing DNA damage in individual cells was exposed under γ-radiation. The increase in the severity of DNA damage was observed with the increase dose (1, 2 and 4Gy) of γ-radiation in cultured hepatocytes. TBARS were increased significantly, whereas the levels of GSH, vitamins C, E and A, ceruloplasmin, uric acid and antioxidant enzymes were significantly decreased in γ-irradiated hepatocytes. The maximum damage to hepatocytes was observed at 4Gy irradiation. On pretreatment with curcumin (1, 5 and 10μg/ml) showed a significant decrease in the levels of TBARS and DNA damage. The antioxidant enzymes were increased significantly along with the levels of GSH, vitamins A, E and C, uric acid and ceruloplamin. The maximum protection of hepatocytes was observed at 10μg/ml of curcumin pretreatment. Thus, pretreatment with curcumin helps in protecting the hepatocytes against γ-radiation-induced cellular damage and can be developed as an effective radioprotector during radiotherapy in near future.
已知电离辐射通过产生活性氧（ROS）诱导氧化应激，导致细胞内促氧化剂和抗氧化剂失衡，这被认为最终会导致细胞死亡。本研究旨在评估姜黄素（姜黄的一种黄色色素）对分离的大鼠原代肝细胞中γ辐射诱导的毒性的辐射防护作用。通过胶原酶灌注从大鼠肝脏中分离肝细胞。使用脂质过氧化指标如硫代巴比妥酸反应物质（TBARS）、抗氧化剂超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）和还原型谷胱甘肽（GSH）、铜蓝蛋白、维生素A、E和C以及尿酸来评估细胞变化。彗星试验是一种用于定量和分析单个细胞中DNA损伤的灵敏且快速的技术，将细胞暴露于γ辐射下。在培养的肝细胞中，随着γ辐射剂量（1、2和4Gy）的增加，观察到DNA损伤严重程度增加。γ辐射的肝细胞中TBARS显著增加，而GSH、维生素C、E和A、铜蓝蛋白、尿酸和抗氧化酶的水平显著降低。在4Gy辐射时观察到对肝细胞的最大损伤。用姜黄素（1、5和10μg/ml）预处理后，TBARS水平和DNA损伤显著降低。抗氧化酶以及GSH、维生素A、E和C、尿酸和铜蓝蛋白的水平显著增加。在10μg/ml姜黄素预处理时观察到对肝细胞的最大保护作用。因此，姜黄素预处理有助于保护肝细胞免受γ辐射诱导的细胞损伤，并且在不久的将来可开发成为放疗期间有效的辐射防护剂。
