Sohrabi Aria, Tehrani Ali Asghar, Asri-Rezaei Siamak, Zeinali Ahad, Norouzi Mehdi
Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
Department of Clinical Pathology and Internal Medicine, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
Vet Res Forum. 2020 Fall;11(4):347-353. doi: 10.30466/vrf.2018.93499.2260. Epub 2020 Dec 15.
Gamma radiation are used in many medical and technical applications, however, it is one of the most dangerous kinds of radiation and can be harmful to the body. The present study was designed to clarify the protective effects of the selenium supplementation as selenium nanoparticle and selenite selenium in rat liver against Gamma irradiation with different intensities of 2.00 and 8.00 Gy. A total number of 45 healthy male Wistar rats were randomly divided into nine groups of five each. The radiation procedure was carried out in the Cobalt 60 equipment in Omid hospital, Urmia. The animals were simultaneously immobilized in a transparent acrylic plate and exposed to different intensities of 2.00 and 8.00 Gy radiations on day 7 and 14 of the experiment. After 72 hr after the last radiation, the animals were euthanized, and blood and liver tissue were collected. Histological analyses revealed the radiation-induced hepatic injury in rats, which included vacuolated cytoplasm, liver necrosis, fibrosis, and vascular lesions followed by a significant increase in alanine transaminase, alanine transaminase, alkaline phosphatase, and Gamma-glutamyl transferase. Selenium nanoparticles bear a more potent antioxidant effect in comparison with selenium selenite and can effectively protect the liver cell against Gamma radiation at a dose of 8.00 Gy.
伽马辐射被用于许多医学和技术应用中，然而，它是最危险的辐射类型之一，可能对身体有害。本研究旨在阐明补充纳米硒和亚硒酸钠形式的硒对大鼠肝脏抵抗2.00和8.00 Gy不同强度伽马辐射的保护作用。总共45只健康雄性Wistar大鼠被随机分为9组，每组5只。辐射程序在乌尔米耶市奥米德医院的钴60设备中进行。在实验的第7天和第14天，将动物同时固定在透明丙烯酸板上，并暴露于2.00和8.00 Gy的不同强度辐射下。在最后一次辐射后72小时，对动物实施安乐死，并采集血液和肝脏组织。组织学分析显示大鼠存在辐射诱导的肝损伤，包括细胞质空泡化、肝坏死、纤维化和血管病变，随后丙氨酸转氨酶、天冬氨酸转氨酶、碱性磷酸酶和γ-谷氨酰转移酶显著升高。与亚硒酸钠相比，纳米硒具有更强的抗氧化作用，并且在8.00 Gy剂量下可以有效保护肝细胞免受伽马辐射。
