CYP1A1 和 MT1K 是多氯联苯诱导肝脏疾病的同类特异性生物标志物基因。
CYP1A1 and MT1K are congener specific biomarker genes for liver diseases induced by PCBs.
机构信息
Molecular Genetics Laboratory, Department of Biology, Howard University, 415 College Street, NW, Washington DC 20059, USA.
出版信息
Environ Toxicol Pharmacol. 2008 Mar;25(2):218-21. doi: 10.1016/j.etap.2007.10.018. Epub 2007 Oct 16.
We have identified two most significant biomarker genes, CYP1A1 (69.81 up-regulation) and MT1K (14.66 up-regulation), showing highest overexpression at p-value <0.005. These were selected out of several hundred genes induced in vitro, using PCB exposed human liver (HepG2) cells. Over expression of the CYP1A1 (cytochrome P450) gene was specific to PCB-77 and MT1K (Metallothionein) to PCB-153. Affymetrix oligonucleotide microarrays (mRNA) were used to screen the entire genome of human liver cells in a time-dependent exposure and were further validated by quantitative real-time RT-PCR.
我们已经鉴定出两个最重要的生物标志物基因，CYP1A1（上调 69.81%）和 MT1K（上调 14.66%），其表达水平在 p 值<0.005 时显著升高。这些基因是从使用 PCB 暴露的人肝（HepG2）细胞体外诱导的数百个基因中筛选出来的。CYP1A1（细胞色素 P450）基因的过度表达是 PCB-77 特异性的，而 MT1K（金属硫蛋白）则是 PCB-153 特异性的。Affymetrix 寡核苷酸微阵列（mRNA）用于在时间依赖性暴露的情况下筛选人肝细胞的整个基因组，并通过实时定量 RT-PCR 进一步验证。
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