Toxicology Unit, Biology Division, Indian Institute of Chemical Technology, Hyderabad 500007, India.
Environ Toxicol Pharmacol. 2008 Sep;26(2):192-8. doi: 10.1016/j.etap.2008.03.010. Epub 2008 Mar 29.
The recovery effect of chlorpyrifos (CPF) on antioxidant enzymes, locomotor behaviour and the target enzyme acetylcholinesterase (AChE) interaction were studied after exposure to 297μgL(-1) (LC(50) for 96h) in mosquito fish, Gambusia affinis. Activities of the antioxidant enzymes-superoxide dismutase, catalase, glutathione reductase in viscera, and AChE in brain were inhibited at 96h of exposure. However, induction in lipid peroxidation was observed. The antioxidant levels were restored to near control by 16-18 days. Similarly, swimming speed and AChE were also recovered but comparatively needs longer period. In vitro AChE study indicated that CPF alters the apparent K(m) values, resulting in a competitive type of inhibition and the inhibitory constant K(i) was found to be 4.57×10(-4)M. The results showed that the organophosphate CPF besides its inhibitory effect on target enzyme AChE also inhibits antioxidant enzymes, which can be used as biomarkers in the pesticide-contaminated aquatic streams.
在 96 小时接触 297μg/L（96 小时 LC50）的情况下，研究了对鱼（Gambusia affinis）中氯蜱硫磷（CPF）对抗氧化酶、运动行为和靶酶乙酰胆碱酯酶（AChE）相互作用的恢复效应。暴露 96 小时后，内脏中超氧化物歧化酶、过氧化氢酶、谷胱甘肽还原酶和大脑中的 AChE 活性受到抑制。然而，观察到脂质过氧化作用增强。在 16-18 天内，抗氧化水平恢复到接近对照水平。同样，游泳速度和 AChE 也得到了恢复，但需要更长的时间。体外 AChE 研究表明，CPF 改变了表观 K(m) 值，导致竞争性抑制，并且发现抑制常数 K(i)为 4.57×10(-4)M。结果表明，有机磷 CPF 除了对靶酶 AChE 的抑制作用外，还抑制抗氧化酶，可作为受农药污染的水溪中的生物标志物。
