Teaching Institute of Public Health, Primorsko-Goranska County, Medical Faculty, University of Rijeka, Rijeka, Croatia.
Environ Toxicol Pharmacol. 2009 Nov;28(3):439-47. doi: 10.1016/j.etap.2009.08.005. Epub 2009 Sep 6.
In an attempt to assess the intensity of environmental pollution in industrial zones of Kvarnerian Bay in Northern Adriatic Sea and the reactivity of Mytilus galloprovincialis to these changes, in this study we estimated the concentration of heavy metals at four locations in both sea-sediment and in the mussels. Further we tried to correlate these changes with seasonal variations in environmental temperature, pH and salinity, as well as with the expression of metallothioneins (MTs) and heat shock proteins (HSPs) in the digestive tract of the mussels. Sampling in vivo was performed monthly, during the year 2008, while under the laboratory conditions the reactivity of acclimated mussels were tested to increasing concentrations of CdCl(2) and to thermal stress. The data have shown that the induction of MTs and HSP isoforms of the 70-kDa size class were highly affected by model agents treatment including contamination of sea-sediment by Pb, Hg and Cd, implying that these stress proteins might be power biomarkers of marine pollution.
为了评估亚得里亚海北部克瓦内尔湾（Kvarnerian Bay）工业区的环境污染程度以及厚壳贻贝（Mytilus galloprovincialis）对这些变化的反应，本研究在四个地点测量了海沉积物和贻贝中的重金属浓度。此外，我们还试图将这些变化与环境温度、pH 值和盐度的季节性变化以及贻贝消化道内金属硫蛋白（MTs）和热休克蛋白（HSPs）的表达进行关联。2008 年，我们每月进行一次体内采样，同时在实验室条件下，用递增浓度的 CdCl2 和热应激来测试驯化贻贝的反应。研究数据表明，模型试剂处理（包括 Pb、Hg 和 Cd 污染海沉积物）对 MTs 和 HSP 同工型 70kDa 大小类别的诱导作用受到高度影响，这意味着这些应激蛋白可能是海洋污染的有力生物标志物。
