I 型、II 型、III 型和 IV 型醇脱氢酶同工酶和醛脱氢酶在宫颈癌中的活性。
The activity of class I, II, III and IV alcohol dehydrogenase isoenzymes and aldehyde dehydrogenase in cervical cancer.
机构信息
Department of Biochemical Diagnostics, Medical University, Bialystok, Poland.
出版信息
Clin Biochem. 2011 Oct;44(14-15):1231-4. doi: 10.1016/j.clinbiochem.2011.07.004. Epub 2011 Jul 18.
OBJECTIVE
The aim of this study was to compare the metabolism of cervical cancer cells and normal cervical cells by measurement of ADH isoenzymes and ALDH activities.
METHODS
The study material consisted of 40 cancerous cervical tissues. Class III, IV of ADH and total ADH activity was measured by the photometric method and class I, II ADH and ALDH activity by the fluorometric method with class-specific fluorogenic substrates.
RESULTS
The activity of the class I ADH isoenzyme and the total ADH were significantly higher in cervical cancer as compared to healthy tissues. Class I of ADH and total ADH activity are significantly higher in every stage of cancer as compared to the control. There are no significant differences between planoepitheliale and adenocarcinoma.
CONCLUSION
The increased activity of total ADH in cervical cancer may be the cause of some metabolic disorders in cancer cells, which may intensify carcinogenesis in this organ.
目的
本研究旨在通过测量 ADH 同工酶和 ALDH 活性来比较宫颈癌和正常宫颈细胞的代谢。
方法
研究材料包括 40 例宫颈癌组织。采用分光光度法测定 III 、 IV 类 ADH 和总 ADH 活性，采用荧光法测定 I 、 II 类 ADH 和 ALDH 活性，采用特异性荧光底物。
结果
与健康组织相比，宫颈癌中 I 类 ADH 同工酶和总 ADH 活性显著升高。与对照组相比，每个癌症阶段的 I 类 ADH 和总 ADH 活性均显著升高。扁平上皮和腺癌之间无显著差异。
结论
宫颈癌中总 ADH 活性的增加可能是癌细胞某些代谢紊乱的原因，这可能会加剧该器官的癌变。
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