Jelski Wojciech, Orywal Karolina, Panek Bogusław, Gacko Marek, Mroczko Barbara, Szmitkowski Maciej
Department of Biochemical Diagnostics, Medical University, Bialystok, Poland.
Exp Mol Pathol. 2009 Aug;87(1):59-62. doi: 10.1016/j.yexmp.2009.03.001. Epub 2009 Mar 28.
Human blood vessels contain a huge amount of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH), which play a significant role in the metabolism of many biological substances and participate in various metabolic pathways. The aim of this study was the investigation of the differences between the activities of ADH and ALDH in the wall of aortic aneurysm and wall of healthy aorta, that can explain the pathological background of aneurysm development.
For the measurement of the activity of class I and II ADH isoenzymes and ALDH activity the fluorometric methods was employed. The total ADH activity and activity of class III and IV isoenzymes was measured by the photometric method. The study material consisted of vessels wall samples obtained from 45 abdominal aortic aneurysm.
The activity of the class I ADH isoenzyme was significantly lower in the wall of aortic aneurysm than in healthy aorta. The other tested classes of ADH showed the tendency to lower level of the activity in aneurysm tissue than that in wall of unchanged aorta. The activities of total ADH and ALDH were also not significantly lower in the aneurysms.
The decrease of the activity of class I ADH isoenzymes in the wall of aortic aneurysm may be a factor of some disorders in metabolic pathways with participation of these isoenzymes.
人体血管中含有大量乙醇脱氢酶（ADH）和乙醛脱氢酶（ALDH），它们在多种生物物质的代谢中起重要作用，并参与各种代谢途径。本研究的目的是调查主动脉瘤壁和健康主动脉壁中ADH和ALDH活性的差异，以解释动脉瘤形成的病理背景。
采用荧光法测定I类和II类ADH同工酶的活性以及ALDH活性。用光度法测定总ADH活性以及III类和IV类同工酶的活性。研究材料包括从45个腹主动脉瘤获取的血管壁样本。
主动脉瘤壁中I类ADH同工酶的活性明显低于健康主动脉。其他检测的ADH类别显示，动脉瘤组织中的活性水平有低于未病变主动脉壁的趋势。动脉瘤中总ADH和ALDH的活性也没有显著降低。
主动脉瘤壁中I类ADH同工酶活性的降低可能是这些同工酶参与的代谢途径中某些紊乱的一个因素。
