醇脱氢酶（ADH）和醛脱氢酶（ALDH）作为胰腺癌患者肿瘤标志物的候选物。
Alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) as candidates for tumor markers in patients with pancreatic cancer.
机构信息
Department of Biochemical Diagnostics, Medical University Bialystok, Poland.
出版信息
J Gastrointestin Liver Dis. 2011 Sep;20(3):255-9.
BACKGROUND
Various alcohol dehydrogenase (ADH) isoenzymes and aldehyde dehydrogenase (ALDH) exist in the pancreas. Moreover, ADH and ALDH are present in pancreatic cancer cells. The activity of ADH class III isoenzymes is significantly higher in cancerous than in healthy tissues. The expression of these enzymes in cancer cells is reflected by increased enzyme activity in the sera and thus could be helpful for diagnosing pancreatic cancer. The aim of this study was to investigate the potential role of ADH and ALDH as tumor markers for pancreatic carcinoma.
METHODS
Serum samples were taken from 165 patients with pancreatic cancer and 166 healthy controls. Total ADH activity and class III and IV isoenzymes were measured by photometric and ALDH activity, ADH I and II by the fluorometric method.
RESULTS
There was a significant increase in the activity of ADH III isoenzyme (14.03 mU/l vs 11.45 mU/l; p < 0.001) and total ADH activity in the sera of pancreatic cancer patients compared to the control. The diagnostic sensitivity for ADH III was 70%, specificity 76%, positive and negative predictive values were 79% and 71% respectively. Area under ROC curve for ADH III was 0.64.
CONCLUSION
The results suggest a potential role for ADH III as a marker of pancreatic cancer.
背景
胰腺中存在多种醇脱氢酶（ADH）同工酶和醛脱氢酶（ALDH）。此外，ADH 和 ALDH 存在于胰腺癌细胞中。III 类 ADH 同工酶在癌症组织中的活性明显高于健康组织。这些酶在癌细胞中的表达反映在血清中酶活性的增加，因此有助于诊断胰腺癌。本研究旨在探讨 ADH 和 ALDH 作为胰腺癌肿瘤标志物的潜在作用。
方法
从 165 例胰腺癌患者和 166 例健康对照者中采集血清样本。通过分光光度法测定总 ADH 活性和 III 型和 IV 型同工酶，通过荧光法测定 ALDH 活性、ADH I 和 II。
结果
与对照组相比，胰腺癌患者血清中 ADH III 同工酶（14.03 mU/l 比 11.45 mU/l；p < 0.001）和总 ADH 活性显著升高。ADH III 的诊断灵敏度为 70%，特异性为 76%，阳性和阴性预测值分别为 79%和 71%。ADH III 的 ROC 曲线下面积为 0.64。
结论
结果表明 ADH III 可能作为胰腺癌的标志物。
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