National Institute of Advanced Industrial Science and Technology, Higashi, Tsukuba 305-8565, Japan.
Carbohydr Res. 2011 Sep 27;346(13):1820-6. doi: 10.1016/j.carres.2011.06.025. Epub 2011 Jun 27.
A series of sugar-modified porous silica monoliths with different sugar ligands (β-lactoside, β-N-acetyllactosaminide, β-d-galactoside, β-d-N-acetylgalactosaminide and β-d-glucoside) and linkers were prepared and evaluated using plant toxins and lectins including ricin and a Ricinus communis agglutinin (RCA(120)). Among these sugar monoliths, a lactose monolith carrying a triethylene glycol spacer adsorbed ricin and RCA(120) with the highest efficiency. The monolith showed no binding with albumin, globulin, and lectins from Jack beans, Osage orange, Amur maackia and wheat germ. All these data support the utility of the lactose-modified monolith as a tool for adsorption and decontamination of plant toxins.
一系列具有不同糖配体（β-乳糖、β-N-乙酰乳糖胺、β-半乳糖、β-N-乙酰半乳糖胺和β-葡萄糖苷）和连接体的糖修饰多孔硅质整体材料被制备并使用植物毒素和凝集素（包括蓖麻毒素和蓖麻凝集素（RCA(120)）进行了评价。在这些糖整体材料中，带有三乙二醇间隔臂的乳糖整体材料对蓖麻毒素和 RCA(120)的吸附效率最高。该整体材料与白蛋白、球蛋白以及来自菜豆、皂荚、密花美登木和麦胚的凝集素没有结合。所有这些数据都支持了乳糖修饰的整体材料作为吸附和净化植物毒素的工具的应用。
