College of Pharmacy, Korea University, Jochiwon, Yeongi, Chungnam 339-700, Republic of Korea.
Biomaterials. 2011 Nov;32(31):7924-31. doi: 10.1016/j.biomaterials.2011.07.017. Epub 2011 Jul 23.
The Pluronic nanoparticles (NPs) composed of Pluronic (F-68) and liquid polyethylene glycol (PEG, molecular wt: 400) containing docetaxel (DTX) were stabilized with the vesicle fusion. When DTX-loaded Pluronic NPs were mixed with vesicles in the aqueous medium, DTX-loaded Pluronic NPs were incorporated into vesicles to form multi-core vesicle NPs. The morphology and size distribution of multi-core vesicle NPs were observed using FE-SEM, cryo-TEM and a particle size analyzer. To apply multi-core vesicle NPs as a delivery system for DTX, a model anti-cancer drug, the release pattern of DTX was observed and the tumor growth was monitored by injecting the DTX-loaded multi-core vesicle NPs into the tail veins of tumor-bearing mice. We also evaluated the time-dependent excretion profile, in vivo biodistribution, circulation time, and tumor targeting capability of multi-core vesicle NPs using a non-invasive live animal imaging technology.
由 Pluronic（F-68）和含有多西紫杉醇（DTX）的液态聚乙二醇（PEG，分子量：400）组成的 Pluronic 纳米颗粒（NPs）通过囊泡融合而稳定。当载有多西紫杉醇的 Pluronic NPs 与水相中的囊泡混合时，载有多西紫杉醇的 Pluronic NPs 被包裹入囊泡中形成多核囊泡 NPs。使用 FE-SEM、 cryo-TEM 和粒径分析仪观察多核囊泡 NPs 的形态和粒径分布。为了将多核囊泡 NPs 作为一种载药系统应用于多西紫杉醇（一种模型抗癌药物），观察了 DTX 的释放模式，并通过向荷瘤小鼠尾静脉注射载有多西紫杉醇的多核囊泡 NPs 来监测肿瘤生长。我们还使用非侵入性活体动物成像技术评估了多核囊泡 NPs 的时间依赖性排泄特征、体内分布、循环时间和肿瘤靶向能力。
