Department of Biochemistry and Cell Biology, Rice University, Houston, TX 77005, USA.
Biomaterials. 2012 Feb;33(6):1821-6. doi: 10.1016/j.biomaterials.2011.11.015. Epub 2011 Dec 2.
Cancer chemotherapies suffer from multi drug resistance, high non-specific toxicity and heterogeneity of tumors. We report a method of plasmonic nanobubble-enhanced endosomal escape (PNBEE) for the selective, fast and guided intracellular delivery of drugs through a self-assembly by cancer cells of separately targeted gold nanoparticles and encapsulated drug (Doxil). The co-localized with Doxil plasmonic nanobubbles optically generated in cancer cells released the drug into the cytoplasm thus increasing the therapeutic efficacy against these drug-resistant cells by 31-fold, reducing drug dose by 20-fold, the treatment time by 3-fold and the non-specific toxicity by 10-fold compared to standard treatment. Thus the PNBEE mechanism provided selective, safe and efficient intracellular drug delivery in heterogeneous environment opening new opportunities for drug therapies.
癌症化疗存在多药耐药性、高非特异性毒性和肿瘤异质性等问题。我们报告了一种等离子体纳米泡增强内体逃逸（PNBEE）的方法，用于通过癌细胞对靶向金纳米颗粒和包裹药物（Doxil）的自组装，选择性、快速和引导性地将药物递送至细胞内。在癌细胞中光学产生的与 Doxil 共定位的等离子体纳米泡将药物释放到细胞质中，从而使这些耐药细胞的治疗效果提高 31 倍，药物剂量降低 20 倍，治疗时间缩短 3 倍，非特异性毒性降低 10 倍，与标准治疗相比。因此，PNBEE 机制在异质环境中提供了选择性、安全和有效的细胞内药物递送，为药物治疗开辟了新的机会。
