Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, China-America CRC for Environment & Health, Shandong Province, 27# Shanda South Road, Jinan 250100, PR China.
J Hazard Mater. 2011 Sep 15;192(3):1766-71. doi: 10.1016/j.jhazmat.2011.07.012. Epub 2011 Jul 8.
The effects of 4-aminoantipyrine (AAP) on oxidative stress induced by glutathione (GSH) depletion in single human erythrocytes were investigated using microfluidic technique and fluorescence imaging. Most cell-based toxicity evaluations on GSH are performed with bulk experiments based on analysis of cell populations. This work established a single-cell toxicity evaluation method to statistically analyze the GSH amount in single erythrocytes incubated with AAP in different concentrations. The experimental conditions of cell flow rate and cell concentration were optimized. The GSH contents in erythrocytes decreased with increasing dose of AAP. At low concentration, AAP had a little effect on GSH; while at high concentration, AAP led to GSH depletion reaching a maximum of 14.53%. The depletion of GSH leads to a significant shift to a more oxidizing intracellular environment. This study provides basic data for presenting the effect of AAP on GSH in erythrocytes and is helpful for understanding its toxicity during the blood transportation process. In addition, it will also complement studies on the environmental risk assessment of AAP pollution.
采用微流控技术和荧光成像技术，研究了 4-氨基安替比林（AAP）对人红细胞谷胱甘肽（GSH）耗竭诱导的氧化应激的影响。大多数基于细胞群体分析的 GSH 细胞毒性评价都是基于批量实验进行的。本工作建立了一种单细胞毒性评价方法，用于统计分析不同浓度 AAP 孵育的单个红细胞中的 GSH 含量。优化了细胞流速和细胞浓度的实验条件。红细胞中的 GSH 含量随 AAP 剂量的增加而降低。在低浓度时，AAP 对 GSH 的影响较小；而在高浓度时，AAP 导致 GSH 耗竭，达到最大值 14.53%。GSH 的耗竭导致细胞内环境向更氧化的方向发生显著变化。该研究为展示 AAP 对红细胞中 GSH 的影响提供了基础数据，有助于了解其在血液运输过程中的毒性。此外，它还将补充对 AAP 污染的环境风险评估的研究。
