Kayan Mustafa, Naziroğlu Mustafa, Celik Omer, Yalman Kadir, Köylü Halis
Department of Radiology, Isparta State Hospital, Isparta, Turkey.
Cell Biochem Funct. 2009 Oct;27(7):424-9. doi: 10.1002/cbf.1589.
X-ray radiation is detrimental to human cells and may lead to development of life-threatening diseases. Cigarette smoke contains about 500 chemicals that include organic and oxidant compounds whereas vitamin C and E (VCE) have scavenger effects on the compounds. We investigated effects of VCE administration on X-ray-induced oxidative toxicity in blood of smoker and nonsmoker X-ray technicians. Twenty technicians and 30 healthy age-matched subjects control were used in the study. Ten of the X-ray technicians and 15 of the control were smokers. Blood samples were taken from the control. Oral vitamin C (500 mg) and vitamin E (150 mg) were daily supplemented to the smoker and nonsmoker X-ray technicians for 5 weeks. Blood samples were taken from the X-ray technicians after and before 5 weeks. Plasma and erythrocytes lipid peroxidation (LP), reduced glutathione (GSH) levels, erythrocytes glutathione peroxidase (GSH-Px), and plasma antioxidant vitamin concentrations were investigated in control and X-ray technicians with smoker and nonsmoker. Plasma and erythrocytes LP levels were higher in the total X-ray group and smoker X-ray group than in control and nonsmoker X-ray group, respectively although the LP level was decreased by the VCE treatment. The plasma vitamin C, vitamin A, vitamin E, and beta-carotene concentrations were lower in the X-ray group than in control although their concentrations were increased by the treatment. The erythrocytes GSH level and GSH-Px activity were found to be higher in the treatment group than in the X-ray group. Plasma GSH level was not found to be different in all group. Reactive oxygen species may play role in the mechanism that has been proposed to explain the biological side effect of X-ray radiation and smoke. VCE prevents the smoke and X-ray-induced oxidative stress to strengthen antioxidant vitamin concentrations in the blood of the technicians.
X射线辐射对人体细胞有害，可能导致危及生命的疾病。香烟烟雾含有约500种化学物质，包括有机化合物和氧化剂化合物，而维生素C和E（VCE）对这些化合物具有清除作用。我们研究了VCE给药对吸烟和不吸烟的X射线技术人员血液中X射线诱导的氧化毒性的影响。本研究使用了20名技术人员和30名年龄匹配的健康对照受试者。其中10名X射线技术人员和15名对照受试者为吸烟者。从对照组采集血样。对吸烟和不吸烟的X射线技术人员每天补充口服维生素C（500毫克）和维生素E（150毫克），持续5周。5周前后从X射线技术人员采集血样。对对照组以及吸烟和不吸烟的X射线技术人员的血浆和红细胞脂质过氧化（LP）、还原型谷胱甘肽（GSH）水平、红细胞谷胱甘肽过氧化物酶（GSH-Px）以及血浆抗氧化维生素浓度进行了研究。尽管VCE治疗降低了LP水平，但总X射线组和吸烟X射线组的血浆和红细胞LP水平分别高于对照组和不吸烟X射线组。X射线组的血浆维生素C、维生素A、维生素E和β-胡萝卜素浓度低于对照组，不过治疗使其浓度有所增加。发现治疗组的红细胞GSH水平和GSH-Px活性高于X射线组。所有组的血浆GSH水平未发现差异。活性氧可能在已提出的解释X射线辐射和烟雾生物学副作用的机制中起作用。VCE可预防烟雾和X射线诱导的氧化应激，以增强技术人员血液中的抗氧化维生素浓度。
