高温古菌中的前病毒诱导：嗜热古菌纺锤形病毒 1 和嗜热古菌卵形病毒 1 的特性。
Provirus induction in hyperthermophilic archaea: characterization of Aeropyrum pernix spindle-shaped virus 1 and Aeropyrum pernix ovoid virus 1.
机构信息
Molecular Biology of the Gene in Extremophiles Unit, Department of Microbiology, Institut Pasteur, rue Dr. Roux 25, 75724 Paris Cedex 15, France.
出版信息
J Bacteriol. 2011 Oct;193(19):5412-9. doi: 10.1128/JB.05101-11. Epub 2011 Jul 22.
Abstract
By in silico analysis, we have identified two putative proviruses in the genome of the hyperthermophilic archaeon Aeropyrum pernix, and under special conditions of A. pernix growth, we were able to induce their replication. Both viruses were isolated and characterized. Negatively stained virions of one virus appeared as pleomorphic spindle-shaped particles, 180 to 210 nm by 40 to 55 nm, with tails of heterogeneous lengths in the range of 0 to 300 nm. This virus was named Aeropyrum pernix spindle-shaped virus 1 (APSV1). Negatively stained virions of the other virus appeared as slightly irregular oval particles with one pointed end, while in cryo-electron micrographs, the virions had a regular oval shape and uniform size (70 by 55 nm). The virus was named Aeropyrum pernix ovoid virus 1 (APOV1). Both viruses have circular, double-stranded DNA genomes of 38,049 bp for APSV1 and 13,769 bp for APOV1. Similarities to proteins of other archaeal viruses were limited to the integrase and Dna1-like protein. We propose to classify APOV1 into the family Guttaviridae.
摘要
通过计算机分析，我们在嗜热古菌 Aeropyrum pernix 的基因组中鉴定出两个推定的前病毒，并在 Aeropyrum pernix 生长的特殊条件下，能够诱导它们复制。这两种病毒都被分离和鉴定。一种病毒的负染病毒体呈多形性纺锤形颗粒，长 180 至 210nm，宽 40 至 55nm，尾部长度在 0 至 300nm 之间不等。这种病毒被命名为 Aeropyrum pernix 纺锤形病毒 1（APSV1）。另一种病毒的负染病毒体呈略微不规则的椭圆形颗粒，一端较尖，而在冷冻电镜照片中，病毒呈规则的椭圆形，大小均匀（70nm×55nm）。这种病毒被命名为 Aeropyrum pernix 卵形病毒 1（APOV1）。这两种病毒都有圆形、双链 DNA 基因组，APSV1 为 38049bp，APOV1 为 13769bp。与其他古病毒蛋白的相似性仅限于整合酶和 Dna1 样蛋白。我们建议将 APOV1 分类为 Guttaviridae 科。
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