钨酸盐在原核 ABC 转运系统中选择性的分子基础。
A molecular basis for tungstate selectivity in prokaryotic ABC transport systems.
机构信息
Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands.
出版信息
J Bacteriol. 2011 Sep;193(18):4999-5001. doi: 10.1128/JB.05056-11. Epub 2011 Jul 22.
Abstract
The essential trace compounds tungstate and molybdate are taken up by cells via ABC transporters. Despite their similar ionic radii and chemical properties, the WtpA protein selectively binds tungstate in the presence of molybdate. Using site-directed mutagenesis of conserved binding pocket residues, we established a molecular basis for tungstate selectivity.
摘要
基本痕量化合物钨酸盐和钼酸盐通过 ABC 转运蛋白被细胞摄取。尽管它们的离子半径和化学性质相似，但在钼酸盐存在的情况下，WtpA 蛋白选择性地结合钨酸盐。通过对保守结合口袋残基的定点突变，我们为钨酸盐的选择性建立了分子基础。
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