Department of Microbiology and Immunology, Cancer Research Institute and Liver Research Institute, College of Medicine, Seoul National University, Seoul 110-799, Republic of Korea.
Department of Laboratory Medicine, Ulsan University Hospital, Ulsan, Republic of Korea.
Int J Syst Evol Microbiol. 2012 Jun;62(Pt 6):1289-1295. doi: 10.1099/ijs.0.033274-0. Epub 2011 Jul 22.
A novel slow-growing, non-chromogenic mycobacterium (strain 01-305(T)) was isolated from a patient with pulmonary dysfunction. Growth characteristics, acid-fastness and the results of 16S rRNA gene sequencing supported the placement of this strain within the genus Mycobacterium. Phenotypically, strain 01-305(T) was generally similar to Mycobacterium triviale ATCC 23292(T), but some unique biochemical characteristics were observed. The 16S rRNA gene sequence of strain 01-305(T) was similar to those of M. triviale ATCC 23290 (GenBank accession no. AY734996, 99.9 % similarity) and M. triviale ATCC 23291 (AY734995, 99.9 %); however, it differed substantially from that of M. triviale ATCC 23292(T) (X88924, 98.2 %). Phylogenetic analysis based on 16S rRNA gene sequences placed strain 01-305(T) in the slow-growing Mycobacterium group close to M. triviale ATCC 23290 and M. triviale ATCC 23291, but not M. triviale ATCC 23292(T). Unique mycolic acid profiles and phylogenetic analysis based on two different chronometer molecules, and the hsp65 and rpoB genes, strongly supported the taxonomic status of this strain as representing a distinct species. These data support the conclusion that strain 01-305(T) represents a novel mycobacterial species, for which the name Mycobacterium koreense sp. nov. is proposed. The type strain is 01-305(T) ( = DSM 45576(T) = KCTC 19819(T)).
一株新型生长缓慢、非产色分枝杆菌（01-305(T) 株）从一位肺部功能障碍患者中分离得到。生长特性、抗酸性和 16S rRNA 基因测序结果支持将该菌株归入分枝杆菌属。表型上，01-305(T) 株与 Mycobacterium triviale ATCC 23292(T) 通常相似，但观察到一些独特的生化特征。01-305(T) 株的 16S rRNA 基因序列与 Mycobacterium triviale ATCC 23290（GenBank 登录号 AY734996，相似度 99.9%）和 Mycobacterium triviale ATCC 23291（AY734995，相似度 99.9%）相似；然而，与 Mycobacterium triviale ATCC 23292(T)（X88924，相似度 98.2%）差异较大。基于 16S rRNA 基因序列的系统发育分析将 01-305(T) 株置于生长缓慢的分枝杆菌群中，与 Mycobacterium triviale ATCC 23290 和 Mycobacterium triviale ATCC 23291 接近，但与 Mycobacterium triviale ATCC 23292(T) 不同。独特的类脂酸图谱以及基于两个不同计时分子（hsp65 和 rpoB 基因）的系统发育分析强烈支持该菌株代表一个独特物种的分类地位。这些数据支持 01-305(T) 株代表一种新型分枝杆菌的结论，建议将其命名为 Mycobacterium koreense sp. nov.。模式菌株为 01-305(T)（=DSM 45576(T)=KCTC 19819(T)）。
