Ward Marsha D W, Chung Yong Joo, Copeland Lisa B, Doerfler Donald L
National Health and Environmental Effects Research Laboratory, U.S. Environmental Protection Agency, 109 T. W. Alexander Drive, MD B105-02, Research Triangle Park, NC 27711, USA.
J Toxicol. 2011;2011:360805. doi: 10.1155/2011/360805. Epub 2011 Jun 21.
Biopesticides can be effective in controlling their target pest. However, research regarding allergenicity and asthma development is limited. We compared the ability of fungal biopesticide Metarhizium anisopliae (MACA) and house dust mite (HDM) extracts to induce allergic responses in BALB/c mice. The extracts were administered by intratracheal aspiration at doubling doses (2.5-80 μg protein) 4X over a four-week period. Three days after the last exposure, serum and bronchoalveolar lavage fluid (BALF) were collected. The extracts' relative allergenicity was evaluated based on response robustness (lowest significant dose response compared to control (0 μg)). MACA induced a more robust serum total IgE response than HDM. However, in the antigen-specific IgE assay, a similar dose of both MACA and HDM was required to achieve the same response level. Our data suggest a threshold dose of MACA for allergy induction and that M. anisopliae may be similar to HDM in allergy induction potential.
生物农药在控制其目标害虫方面可能有效。然而，关于致敏性和哮喘发展的研究有限。我们比较了真菌生物农药绿僵菌（MACA）和屋尘螨（HDM）提取物在BALB/c小鼠中诱导过敏反应的能力。提取物通过气管内吸入以加倍剂量（2.5 - 80μg蛋白质）在四周内给药4次。最后一次暴露后三天，收集血清和支气管肺泡灌洗液（BALF）。基于反应强度（与对照组（0μg）相比的最低有效剂量反应）评估提取物的相对致敏性。MACA诱导的血清总IgE反应比HDM更强。然而，在抗原特异性IgE测定中，需要相似剂量的MACA和HDM才能达到相同的反应水平。我们的数据表明MACA诱导过敏的阈值剂量，并且绿僵菌在诱导过敏的潜力方面可能与HDM相似。
