Chakraborty Subhankari Prasad, Karmahapatra Santanu, Sahu Sumanta Kumar, Pramanik Panchanan, Roy Somenath
Immunology and Microbiology Laboratory, Department of Human Physiology with Community Health, Vidyasagar University, West Bengal, Midnapore 721 102, India.
Patholog Res Int. 2011;2011:420198. doi: 10.4061/2011/420198. Epub 2011 Jul 20.
Objective. The aim of the present study was to evaluate the possible antioxidant effects of nanoconjugated vancomycin against VRSA infection on select makers of oxidative damage and antioxidant status in spleen. Methods. A coagulase-positive VRSA strain was used for this study. VRSA infection was developed in Swiss mice by intraperitoneal injection of 5 × 10(6) CFU/mL bacterial solutions. VRSA-infected mice were treated with nanoconjugated vancomycin at its effective dose for 10 days. After decapitation, blood was used for determination of viable bacteria count and spleen was excised from control and experimental groups, homogenized and used for different biochemical estimations. Results. Nitrate level, myeloperoxidase activity, lipid peroxidation, protein oxidation, oxidized glutathione, and DNA fragmentation level were increased significantly (P < 0.05) in spleen of VRSA-infected group as compared to control group, and reduced glutathione level, activity of SOD, CAT, GPx, GR, and GST were decreased significantly (P < 0.05); which were increased or decreased significantly (P < 0.05) near to normal in nanoconjugated vancomycin-treated group. Conclusion. These findings suggest the potential use and beneficial role of nanoconjugated vancomycin against VRSA-infection-induced oxidative stress and DNA damage in spleen.
目的。本研究的目的是评估纳米共轭万古霉素对VRSA感染的抗氧化作用，及其对脾脏氧化损伤相关指标和抗氧化状态的影响。方法。本研究使用一株凝固酶阳性的VRSA菌株。通过腹腔注射5×10(6) CFU/mL的细菌溶液，在瑞士小鼠中诱发VRSA感染。用纳米共轭万古霉素的有效剂量对感染VRSA的小鼠进行治疗，持续10天。断头后，取血用于测定活菌数，从对照组和实验组切除脾脏，匀浆后用于不同的生化检测。结果。与对照组相比，VRSA感染组脾脏中的硝酸盐水平、髓过氧化物酶活性、脂质过氧化、蛋白质氧化、氧化型谷胱甘肽和DNA片段化水平显著升高（P < 0.05），而还原型谷胱甘肽水平、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）、谷胱甘肽还原酶（GR）和谷胱甘肽S-转移酶（GST）的活性显著降低（P < 0.05）；在纳米共轭万古霉素治疗组中，这些指标接近正常水平，显著升高或降低（P < 0.05）。结论。这些发现表明纳米共轭万古霉素在对抗VRSA感染诱导的脾脏氧化应激和DNA损伤方面具有潜在用途和有益作用。
