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基于核苷酸修饰 DNA 探针连接的实时 PCR 法超灵敏定量成熟 microRNAs。

Ultrasensitive quantification of mature microRNAs by real-time PCR based on ligation of a ribonucleotide-modified DNA probe.

机构信息

Key Laboratory of Medicine Chemistry and Molecular Diagnosis, Ministry of Education, College of Chemistry and Environment Science, Hebei University, Baoding 071002, Hebei Province, PR China.

出版信息

Chem Commun (Camb). 2011 Sep 7;47(33):9465-7. doi: 10.1039/c1cc13466c. Epub 2011 Jul 22.

DOI:10.1039/c1cc13466c
PMID:21785758
Abstract

It was first demonstrated that the DNA probe modified with ribonucleotides can be efficiently ligated by using miRNA as the template. With PCR amplification of the ligated DNA probe, as low as 0.2 fM target miRNAs can be detected with high specificity.

摘要

首次证明，经核苷酸修饰的 DNA 探针可以通过 miRNA 作为模板进行有效的连接。通过连接 DNA 探针的 PCR 扩增，可以以高特异性检测低至 0.2 fM 的靶标 miRNAs。

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