Department of Molecular and Cell Biology, University of Cape Town, Private Bag 7701, Rondebosch, South Africa.
Mol Biol Rep. 2012 Apr;39(4):4039-49. doi: 10.1007/s11033-011-1185-4. Epub 2011 Jul 23.
Plants activate an array of defence responses following recognition of pathogenic organisms. This study attempted to characterize at a transcriptional level, the defence responses of Arabidopsis thaliana after infection with Botrytis cinerea using microarrays. Alteration in transcript levels following infection was investigated in time (temporal) and space (spatial). A number of genes were up- and down-regulated specifically at 12 h, others at 24 h while others were up- and down-regulated at both time points. Similarly, some genes were specifically induced very close to the lesion while others in more distal tissue. Clustering of expression profiles resulting from other biotic and abiotic interactions with Arabidopsis indicated a large overlap in gene expression. This study highlighted a multitude of genes induced in Arabidopsis spatially and temporally following infection with B. cinerea providing an insight into key processes of defence against this pathogen. The plethora of altered genes identified are candidates for further investigation.
植物在识别病原体后会激活一系列防御反应。本研究试图利用微阵列技术在转录水平上对拟南芥感染灰葡萄孢后的防御反应进行特征描述。通过时间（时间）和空间（空间）来研究感染后转录水平的变化。一些基因在 12 小时时被上调和下调，另一些基因在 24 小时时被上调和下调，而另一些基因在两个时间点都被上调和下调。同样，一些基因在靠近病变的地方特异性诱导，而另一些基因在更远的组织中诱导。来自拟南芥与其他生物和非生物相互作用的表达谱聚类表明，基因表达有很大的重叠。本研究突出了在感染灰葡萄孢后拟南芥在时空上诱导的大量基因，为防御这种病原体的关键过程提供了深入了解。大量鉴定的改变基因是进一步研究的候选基因。
