Iwata N, Inazu N, Satoh T
Department of Pharmacology and Toxicology, Tokyo College of Pharmacy, Japan.
Biol Reprod. 1990 Jan;42(1):161-6. doi: 10.1095/biolreprod42.1.161.
Carbonyl reductase activity and content in the rat ovary were measured at various stages of the estrous cycle, and the enzyme protein in the ovary was localized by immunohistochemistry. The enzyme activity increased after the preovulatory surge of luteinizing hormone (LH) on proestrus, and the enzyme content began to increase prior to the LH surge. Although the enzyme content reached the highest level at 2000 h and remained at a plateau for 8 h, the enzyme activity increased linearly until it reached the highest level at 0800 h on the morning of estrus. At their maximum, enzyme activity and content were approximately 1.5-fold and 2-fold greater, respectively, then basal diestrus values. The enzyme protein amounted to 1-4% of the ovarian cytosolic protein. An immunohistochemical study revealed that the enzyme was primarily localized in interstitial gland cells and theca interna cells of secondary and Graafian follicles as well as atretic follicles.
在动情周期的不同阶段测定大鼠卵巢中的羰基还原酶活性和含量，并通过免疫组织化学对卵巢中的酶蛋白进行定位。在动情前期促黄体生成素（LH）排卵前激增后，酶活性增加，并且酶含量在LH激增之前就开始增加。尽管酶含量在2000 h达到最高水平并在8 h内保持稳定，但酶活性一直呈线性增加，直到在发情日上午0800 h达到最高水平。酶活性和含量在最大值时分别比动情间期基础值大约高1.5倍和2倍。酶蛋白占卵巢胞质蛋白的1-4%。免疫组织化学研究表明，该酶主要定位于间质腺细胞、次级卵泡和格拉夫卵泡的内膜细胞以及闭锁卵泡中。
