Murakami M, Inoshima Y, El-Dakhly Kh M, Yanai T, Ishiguro N
Laboratory of Veterinary Pathology, Department of Veterinary Medicine, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
J Parasitol. 2011 Dec;97(6):1178-80. doi: 10.1645/GE-2830.1. Epub 2011 Jul 25.
Protostrongylus shiozawai (Nematoda: Strongylida) infection is known to occur in the lungs of wild Japanese serows (Capricornis crispus) and, to date, has been classified only by its morphological characteristics, as well as host specificity. To characterize P. shiozawai genetically, a partial sequence of the second internal transcribed spacer (ITS-2) region was determined and compared with those of related nematodes. Our subsequent classification of P. shiozawai based on phylogenetic analysis was consistent with the current classification. Phylogenetic analysis also showed that P. shiozawai is more closely related to Protostrongylus stilesi than to Protostrongylus rufescens. PCR using genetic markers in the ITS-2 region should provide a useful tool for expanded studies of P. shiozawai and other Protostrongylus species.
已知盐泽原圆线虫（线虫纲：圆线目）感染发生在野生日本鬣羚（日本髭羚）的肺部，迄今为止，仅根据其形态特征以及宿主特异性进行分类。为了从基因上表征盐泽原圆线虫，测定了第二内部转录间隔区（ITS-2）区域的部分序列，并与相关线虫的序列进行了比较。我们随后基于系统发育分析对盐泽原圆线虫的分类与当前分类一致。系统发育分析还表明，盐泽原圆线虫与斯氏原圆线虫的关系比与红原圆线虫的关系更密切。使用ITS-2区域的遗传标记进行PCR应为盐泽原圆线虫和其他原圆线虫物种的扩展研究提供有用的工具。
