Faculty of Medicine, Department of Pharmacology and Biochemistry Mu'tah University, Al Karak, Jordan.
J Biomed Sci. 2011 Jul 25;18(1):50. doi: 10.1186/1423-0127-18-50.
Colorectal carcinoma is a common and often fatal disease in which methods of early detection and monitoring are essential. The present study was conducted for measuring serum levels of nucleosomes, carcinoembryonic antigen (CEA) and CA 19-9 in patients newly diagnosed with colorectal carcinoma and confirmed by clinicopathological study.
Thirty subjects were included in the current study: six normal subjects as a control group with mean age (45.6 ± 7.9) and twenty four colorectal carcinoma patients with mean age (46.9 ± 15.6), which were classified pathologically according to the degree of malignant cell differentiation into well differentiated (group I), moderately differentiated (group II) and poorly differentiated (group III). Fasting venous blood samples were collected preoperative.
The results revealed a significant increase in serum level of nucleosomes in patients with poorly differentiated tumors versus patients with well differentiated tumors (p = 0.041). The levels of CEA and CA19-9 showed no significant increase (p = 0.569 and 0.450, respectively).
In conclusion, serum level of nucleosomes provides a highly sensitive and specific apoptotic marker for colorectal carcinoma.
结直肠癌是一种常见且常致命的疾病，早期检测和监测方法至关重要。本研究旨在测量新诊断为结直肠癌患者的血清核小体、癌胚抗原（CEA）和 CA19-9 水平，并通过临床病理研究进行证实。
本研究纳入 30 名受试者：6 名正常受试者作为对照组，平均年龄（45.6±7.9）岁，24 名结直肠癌患者，平均年龄（46.9±15.6）岁，根据恶性细胞分化程度分为高分化（I 组）、中分化（II 组）和低分化（III 组）。术前采集空腹静脉血样本。
结果显示，低分化肿瘤患者的血清核小体水平显著升高，与高分化肿瘤患者相比（p=0.041）。CEA 和 CA19-9 水平无显著升高（p=0.569 和 0.450）。
总之，血清核小体水平为结直肠癌提供了一种高度敏感和特异的凋亡标志物。
