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缺氧促进人牙髓细胞矿化。

Hypoxia promotes mineralization of human dental pulp cells.

机构信息

Department of General Dentistry, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai, China.

出版信息

J Endod. 2011 Jun;37(6):799-802. doi: 10.1016/j.joen.2011.02.028. Epub 2011 Apr 9.

Abstract

INTRODUCTION

Dental pulp can be exposed to hypoxic conditions in case of trauma or inflammation. Dental pulp cells (DPCs) have mineralization potential, which plays a key role in pulp repair and reparative dentinogenesis process. Little information is available about DPC mineralization in hypoxic condition. The purpose of this study was to assess the influence of hypoxia on DPC mineralization to pave the way for a better understanding of dental pulp regeneration and reparative dentin formation.

METHODS

Human DPCs were obtained by using tissue explant technique in vitro and cultured in normoxia (20% O(2)) or hypoxia (5% O(2)). Cell viability was investigated by methyl-thiazol-tetrazolium assay. Cell mineralization was assessed by von Kossa staining and alizarin red S staining. Important mineral genes such as osteocalcin (OCN), dentin matrix acidic phosphoprotein-1 (DMP-1), bone sialoprotein (BSP), and dentin sialophosphoprotein (DSPP) were determined by real-time polymerase chain reaction.

RESULTS

Cell viability of DPCs increased more in hypoxia than in normoxia from day 3 to day 5. Von Kossa staining and alizarin red S staining showed DPCs in hypoxia had higher mineralization activity than in normoxia. Expression of mRNAs for OCN, DMP-1, BSP, and DSPP was greater in hypoxia than in normoxia.

CONCLUSIONS

These results imply that hypoxia promotes DPC mineralization.

摘要

简介

在创伤或炎症的情况下,牙髓可能会暴露在缺氧环境中。牙髓细胞(DPC)具有矿化潜力,这在牙髓修复和修复性牙本质形成过程中起着关键作用。关于缺氧条件下 DPC 矿化的信息很少。本研究旨在评估缺氧对 DPC 矿化的影响,为更好地理解牙髓再生和修复性牙本质形成奠定基础。

方法

采用组织外植体技术体外获取人牙髓细胞,在常氧(20% O(2))或缺氧(5% O(2))条件下培养。通过甲基噻唑四唑测定法检测细胞活力。通过 von Kossa 染色和茜素红 S 染色评估细胞矿化。通过实时聚合酶链反应测定骨钙素(OCN)、牙本质基质酸性磷酸蛋白-1(DMP-1)、骨唾液蛋白(BSP)和牙本质涎磷蛋白(DSPP)等重要矿化基因的表达。

结果

从第 3 天到第 5 天,DPCs 在缺氧条件下的细胞活力比常氧条件下增加得更多。von Kossa 染色和茜素红 S 染色显示,缺氧条件下的 DPCs 具有更高的矿化活性。与常氧相比,缺氧条件下 OCN、DMP-1、BSP 和 DSPP 的 mRNA 表达更高。

结论

这些结果表明缺氧促进了 DPC 的矿化。

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