Xing Houjuan, Wang Jintao, Li Jinlong, Fan Zhaoting, Wang Min, Xu Shiwen
College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Harbin 150030, PR China.
Environ Toxicol Pharmacol. 2010 Jul;30(1):26-30. doi: 10.1016/j.etap.2010.03.009. Epub 2010 Mar 16.
The aim of this study was to investigate the effects of chlorpyrifos (CPF), atrazine (ATR) and the mixture of them on acetylcholinesterase (AChE) and carboxylesterase (CbE) in brain and muscle of common carp, respectively. 220 carps were averagely divided into 11 groups according to the different treatments and concentration, including the exposure and recovery experiments. The activities of AChE and CbE of the brain and muscle were determined at the end of the exposure and the recovery. The results showed that in the control group, the specific enzymatic activities in the brain were higher than that in the muscle. The activities of AChE and CbE in the exposure groups were significantly lower than that in the control group except for the CbE activity in the ATR low-dose group. There was a negative dose-response relationship between the activities of AChE and CbE and the pesticides concentration. The activities of AChE and CbE in the recovery groups were significantly higher than that in the exposure group except for the CbE activity in the ATR low-dose group, AChE activity in the high-dose group of the mixture of ATR and CPF, and AChE activity of the brain in the CPF high-dose group. The results suggested that: (1) brain AChE may be considered as a very sensitive and early biomarker of exposure to CPF, ATR, or the mixture of ATR and CPF, (2) brain CbE may be used as a secondary biomarker for evaluating the exposure to CPF, ATR, or the mixture of ATR and CPF and (3) the change of the AChE and CbE activities caused by the mixture of ATR and CPF was more sensitive than that caused by the ATR or CPF alone.
本研究旨在分别探讨毒死蜱（CPF）、莠去津（ATR）及其混合物对鲤鱼脑和肌肉中乙酰胆碱酯酶（AChE）和羧酸酯酶（CbE）的影响。根据不同处理和浓度，将220尾鲤鱼平均分为11组，包括暴露和恢复实验。在暴露和恢复结束时测定脑和肌肉中AChE和CbE的活性。结果表明，在对照组中，脑中的特异性酶活性高于肌肉中的。除ATR低剂量组的CbE活性外，暴露组中AChE和CbE的活性均显著低于对照组。AChE和CbE的活性与农药浓度之间存在负剂量反应关系。除ATR低剂量组的CbE活性、ATR与CPF混合物高剂量组的AChE活性以及CPF高剂量组脑的AChE活性外，恢复组中AChE和CbE的活性均显著高于暴露组。结果表明：（1）脑AChE可被视为暴露于CPF、ATR或ATR与CPF混合物的非常敏感的早期生物标志物；（2）脑CbE可作为评估暴露于CPF、ATR或ATR与CPF混合物的次要生物标志物；（3）ATR与CPF混合物引起的AChE和CbE活性变化比单独的ATR或CPF引起的变化更敏感。
