College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Harbin 150030, PR China.
Ecotoxicol Environ Saf. 2010 Oct;73(7):1666-70. doi: 10.1016/j.ecoenv.2010.07.013. Epub 2010 Aug 8.
The uses of pesticides and herbicides have become an integral part of modern agricultural systems. The intensive use of pesticides chlorpyrifos (CPF) and herbicides atrazine (ATR) has resulted in serious environmental problems. Herein, we have developed real-time quantitative polymerase chain reaction assays for common carp (Cyprinus carpio L.) mRNA. The levels of AChE mRNA were evaluated in brain and muscle collected from common carp by treatment of ATR, CPF, and their mixture. The decreased transcription of AChE was detected in both tissues at different doses of the toxicants in the end of exposure tests, and the changes were improved in the end of recovery tests in varying degrees. It is suggested that transcription inhibition of AChE might be significant in long-playing single or associated exposure of ATR and CPF in common carp. Alteration in transcription of AChE caused by ATR, CPF, and their mixture could reveal the toxic mechanisms related to cholinergic signaling.
农药和除草剂的使用已成为现代农业系统的一个组成部分。由于大量使用毒死蜱（CPF）和莠去津（ATR），导致了严重的环境问题。在此，我们针对鲤鱼（Cyprinus carpio L.）mRNA 开发了实时定量聚合酶链反应检测方法。通过用 ATR、CPF 及其混合物处理鲤鱼，评估了 ATR、CPF 在脑和肌肉中的 AChE mRNA 水平。在暴露试验结束时，不同剂量的毒物在两种组织中均检测到 AChE 的转录减少，在不同程度的恢复试验结束时，这些变化有所改善。这表明，ATR 和 CPF 的单一或联合长期暴露可能会显著抑制 AChE 的转录。ATR、CPF 及其混合物引起的 AChE 转录改变可能揭示了与胆碱能信号相关的毒性机制。
