Department of Marine Biology, Ocean University of China, Qingdao 266003, China; Research Center for Marine Biology, Tohoku University, Asamushi, Aomori, Aomori 039-3501, Japan.
Environ Toxicol Pharmacol. 2010 Sep;30(2):181-7. doi: 10.1016/j.etap.2010.06.001. Epub 2010 Jun 10.
The neurotoxicity of monocrotophos (MCP) in the development of the serotonergic nervous system and swimming activity of larvae of the sea urchin, Hemicentrotus pulcherrimus, was examined. Lethal dose 50% of MCP was 43μg/ml. Overall morphology was not affected in larvae that received up to 30μg/ml of MCP soon after fertilization until the 53h post-fertilization pluteus stage. However, while 70±0.6% of larvae in 5μg/ml MCP swam actively, the proportion decreased to 30±1.7% in 30μg/ml MCP. Accordingly, immunoblotting indicated that MCP decreased the relative intensity of immunoreaction of serotonin receptor protein. Whole-mount immunohistochemistry indicated that MCP inhibited serotonergic axon growth, reduced the number of serotonergic cells at the apical ganglion, and perturbed formation of the serotonin receptor cell network. The present study demonstrated that sea urchin larva is a useful model for evaluating the working mechanism of environmental toxicants in neurogenesis and behavior.
我们研究了久效磷（MCP）对海洋海胆幼体（Hemicentrotus pulcherrimus）的 5-羟色胺能神经系统发育和游泳活动的神经毒性。MCP 的半数致死剂量为 43μg/ml。在受精后不久，胚胎接受 30μg/ml 以下的 MCP 时，其整体形态没有受到影响，直到受精后 53 小时的幼体盘状幼体阶段。然而，在 5μg/ml MCP 中，70±0.6%的幼虫能够活跃地游泳，而在 30μg/ml MCP 中，这一比例下降到 30±1.7%。相应地，免疫印迹表明 MCP 降低了 5-羟色胺受体蛋白免疫反应的相对强度。全组织免疫组织化学表明，MCP 抑制了 5-羟色胺能轴突的生长，减少了顶端神经节中 5-羟色胺能细胞的数量，并扰乱了 5-羟色胺能受体细胞网络的形成。本研究表明，海洋海胆幼体是评估环境毒物在神经发生和行为中作用机制的有用模型。
