Department of Human Nutrition, Faculty of Food Technology, Agricultural University of Kraków, ul. Balicka 122, 30-149 Kraków, Malopolska, Poland.
Environ Toxicol Pharmacol. 2010 Nov;30(3):245-50. doi: 10.1016/j.etap.2010.06.006. Epub 2010 Jul 7.
The objective of the study was to assess the effect of CLA on serum lipid profile, plasma malondialdehyde and liver histology in Wistar rats fed high-fructose diet. Eighteen rats were randomly assigned to three experimental groups and fed for the next 21 days. The experimental diets were: I, Control; II, Fructose (63.2% of fructose); and III, CLA+Fructose (1% CLA and 63.2% of fructose). The experimental treatments had no effect on body weight of the rats. The LDL+VLDL cholesterol, TG and liver weight were significantly increased in animals fed Fructose. MDA concentrations were significantly increased in rats fed Fructose diet but CLA+Fructose diet had no effect on this marker. In the same line, the histological examination of the livers showed a series of morphological alterations, notably hepatic steatosis in animals fed high-fructose diet. No signs of the steatosis in rats fed CLA+Fructose diet were observed. In conclusion, CLA in high-fructose diet, decreases serum LDL+VLDL and TG and plasma MDA concentrations as well as liver weight and liver cholesterol, thus opposing the effects of high-fructose diet and showing a potential antiatherogenic effect. Similarly, dietary CLA fed at 1% level (w/w) in high-fructose diet, prevented steatosis observed histologically in livers of rats fed high-fructose diets.
本研究旨在评估 CLA 对高果糖饮食喂养的 Wistar 大鼠血清脂质谱、血浆丙二醛和肝组织学的影响。18 只大鼠被随机分配到三个实验组，并在接下来的 21 天内进行喂养。实验饮食为：I，对照；II，果糖（果糖的 63.2%）；和 III，CLA+果糖（1%CLA 和 63.2%果糖）。实验处理对大鼠体重没有影响。喂养果糖的动物 LDL+VLDL 胆固醇、TG 和肝重显著增加。MDA 浓度在喂养果糖饮食的大鼠中显著增加，但 CLA+果糖饮食对该标志物没有影响。同样，肝脏的组织学检查显示出一系列形态改变，特别是在喂养高果糖饮食的动物中出现肝脂肪变性。在喂养 CLA+果糖饮食的大鼠中未观察到脂肪变性的迹象。总之，CLA 在高果糖饮食中可降低血清 LDL+VLDL 和 TG 以及血浆 MDA 浓度以及肝重和肝胆固醇，从而拮抗高果糖饮食的作用并显示出潜在的抗动脉粥样硬化作用。同样，在高果糖饮食中以 1%（w/w）的水平喂养膳食 CLA 可预防在喂养高果糖饮食的大鼠肝脏中观察到的组织学脂肪变性。
