The School of Public Health, Xuzhou Medical College, 84 West Huai-hai Road, Xuzhou, Jiangsu 221002, China.
Environ Toxicol Pharmacol. 2010 Nov;30(3):251-6. doi: 10.1016/j.etap.2010.06.007. Epub 2010 Jul 13.
To study adverse effects and underlying mechanisms of beta-cypermethrin (β-cyp) on male reproductive system, the 15-day intact male adult Sprague-Dawley (SD) rats assay was used as an in vivo test. Male adult SD rats were treated by oral gavage with 0, 15 and 30mgβ-cyp/kgBW for 15 days. After 15-day treatments, the testes, epididymis and seminal vesicles were excised and weighed, respectively. One testis was used for testicular sperm head counts, and the other was for immunohistochemistry test to characterize the expression of androgen receptors (ARs). There were substantial decreases of both sperm head counts and daily sperm production after β-cyp exposure. The expression of AR decreased significantly in rats treated with 15 and 30mgβ-cyp/kgBW, and the gray scale pixel values in the three groups (0, 15 and 30mgβ-cyp/kgBW) were 113.79±13.58, 96.09±5.95 and 77.27±5.44, respectively. These findings suggested β-cyp has significant adverse effects on the reproductive system. Reducing the expression of AR is a potential mechanism of decreased sperm production caused by β-cyp.
为了研究β-氯氰菊酯（β-cyp）对雄性生殖系统的不良影响和潜在机制，采用 15 天完整雄性成年 SD 大鼠试验作为体内试验。雄性成年 SD 大鼠经口灌胃给予 0、15 和 30mgβ-cyp/kgBW，连续 15 天。15 天处理后，分别切除睾丸、附睾和精囊并称重。一侧睾丸用于精子头计数，另一侧用于免疫组织化学试验以表征雄激素受体（ARs）的表达。β-cyp 暴露后精子头计数和每日精子产量均显著下降。用 15 和 30mgβ-cyp/kgBW 处理的大鼠 AR 表达显著降低，三组（0、15 和 30mgβ-cyp/kgBW）的灰度像素值分别为 113.79±13.58、96.09±5.95 和 77.27±5.44。这些发现表明β-cyp 对生殖系统有显著的不良影响。降低 AR 的表达可能是β-cyp 导致精子生成减少的潜在机制。
