Mettler L, Radzun H J, Salmassi A, Köchling W, Parwaresch M R
Department of Obstetrics and Gynecology, Christian-Albrechts-University of Kiel, Kiel, Federal Republic of Germany.
Cancer. 1990 Apr 1;65(7):1525-32. doi: 10.1002/1097-0142(19900401)65:7<1525::aid-cncr2820650713>3.0.co;2-j.
Six monoclonal antibodies directed against ovarian adenocarcinoma were generated by use of 100,000 x g supernatants of Triton-X-100 solubilized extracts of ovarian serous adenocarcinoma as the antigen source. Immunoperoxidase preparation of frozen-sections and routinely processed paraffin section specimens revealed a highly restricted reactivity of these antibodies when tested with adult (n = 2) and fetal (n = 3) tissue types. Coreactivities were occasionally observed with epithelia of the kidney, mammary gland, and pancreas. One monoclonal antibody, Ki-OC I-6-2, cross-reacted only with epididymal epithelia. No coreaction occurred with normal tissue of the ovary, Fallopian tube, or uterus. All antibodies were additionally tested on 74 cases of nonovarian malignancies, 15 cases of ovarian metastases of nonovarian carcinomas, and 114 specimens of ovarian neoplasms other than carcinomas. Ki-OC I-6-2 had no cross-reactivity with these tumors except for one case of renal cell carcinoma. This monoclonal antibody recognized serous, mucinous, and poorly differentiated adenocarcinoma cell types. None of the six antibodies reacted with clear cell or endometrioid carcinoma. All were found to be of the IgG-1 subclass. The tumor antigen to which Ki-OC I-6-2 immunoreacted was estimated to have a molecular weight of 80 kilodaltons (KD).
以卵巢浆液性腺癌经Triton-X-100溶解提取物的100,000 x g上清液为抗原来源，制备了6种针对卵巢腺癌的单克隆抗体。对冷冻切片和常规处理的石蜡切片标本进行免疫过氧化物酶制备后发现，在用成人（n = 2）和胎儿（n = 3）组织类型进行检测时，这些抗体的反应性高度受限。偶尔在肾、乳腺和胰腺的上皮细胞中观察到交叉反应。一种单克隆抗体Ki-OC I-6-2仅与附睾上皮细胞发生交叉反应。卵巢、输卵管或子宫的正常组织未出现交叉反应。所有抗体还在74例非卵巢恶性肿瘤、15例非卵巢癌的卵巢转移瘤以及114例非癌性卵巢肿瘤标本上进行了检测。除1例肾细胞癌外，Ki-OC I-6-2与这些肿瘤均无交叉反应。该单克隆抗体可识别浆液性、黏液性和低分化腺癌细胞类型。这6种抗体均不与透明细胞癌或子宫内膜样癌发生反应。所有抗体均被发现属于IgG-1亚类。Ki-OC I-6-2免疫反应所针对的肿瘤抗原估计分子量为80千道尔顿（KD）。
