Sakakibara K, Ueda R, Ohta M, Nakashima N, Tomoda Y, Takahashi T
Department of Obstetrics and Gynecology, Nagoya University School of Medicine, Japan.
Cancer Res. 1988 Aug 15;48(16):4639-45.
Three mouse IgG1 monoclonal antibodies (MoAbs) reactive predominantly with cytoplasmic antigens of mucinous type ovarian tumors were produced. OM-A MoAb was reactive with 11 of 14 mucinous type and two of three endometrioid type ovarian tumors, although only a minor population of the tumor cells was positive in the latter type. This MoAb was not reactive with serous type, clear cell type, or other types of ovarian tumors, nor with various types of uterine carcinoma. Normal adult and fetal tissues of female genital organs were not positive with this MoAb. Among nongynecological carcinomas, three of six metastatic tumors to the ovary from the gastrointestinal tract, one of five gastric carcinomas, and one of eight lung adenocarcinomas were positive. As for normal adult and fetal tissues of nongynecological origin, epithelium of the normal stomach, small bowel, and bronchus as well as epithelium of fetal small and large bowel and secretory products were weakly positive. Thus, this MoAb showed a selected specificity against mucinous and endometrioid types of ovarian tumors. OM-B MoAb showed a broader specificity than OM-A, reacting with all mucinous type, two of three endometrioid type, and three of 16 serous type ovarian tumors, but not with clear cell type tumors. Adenoma type, but not squamous type, cervical carcinomas and one-half of endometrial carcinomas were positive. This antigen is present in cervical mucosa, but not in ovary or endometrium. OM-C MoAb showed a specificity similar to, but broader than that of, OM-B; i.e., 11 of 14 mucinous type, two of three endometrioid type, nine of 16 serous type, and one of nine clear cell type ovarian tumors were positive. It is reactive with adenoma type uterine carcinoma and normal mucosa of the uterine cervix and with normal surface epithelium of the oviduct. Among nongynecological tumors, OM-B antigen was present in metastatic tumors to the ovary as well as in gastric and pancreatic carcinomas, while OM-C was in metastatic tumors to the ovary and gastric and colonic carcinomas. Thus, the serological analysis showed that these three MoAbs showed selective specificities to mucinous and endometrioid types of ovarian tumors. Preliminary characterization of these three OM antigens suggested that these are distinct from carcinoembryonic antigen or ABH blood group-related antigens.
制备了三种主要与黏液性卵巢肿瘤细胞质抗原发生反应的小鼠IgG1单克隆抗体（MoAb）。OM - A单克隆抗体与14例黏液性肿瘤中的11例以及3例子宫内膜样肿瘤中的2例发生反应，不过在后一种类型中只有少数肿瘤细胞呈阳性。该单克隆抗体与浆液性、透明细胞性或其他类型的卵巢肿瘤均无反应，与各类子宫癌也无反应。正常成年女性和胎儿的女性生殖器官组织对此单克隆抗体均呈阴性。在非妇科癌症中，6例胃肠道转移至卵巢的肿瘤中有3例、5例胃癌中有1例以及8例肺腺癌中有1例呈阳性。至于非妇科来源的正常成年和胎儿组织，正常胃、小肠和支气管的上皮以及胎儿小肠和大肠的上皮及其分泌产物呈弱阳性。因此，该单克隆抗体对黏液性和子宫内膜样类型的卵巢肿瘤具有选择性特异性。OM - B单克隆抗体的特异性比OM - A更广，它与所有黏液性肿瘤、3例子宫内膜样肿瘤中的2例以及16例浆液性肿瘤中的3例发生反应，但与透明细胞性肿瘤无反应。腺瘤型宫颈癌呈阳性，鳞状型宫颈癌阴性，且一半的子宫内膜癌呈阳性。这种抗原存在于宫颈黏膜中，但不存在于卵巢或子宫内膜中。OM - C单克隆抗体显示出与OM - B相似但更广泛的特异性；即14例黏液性肿瘤中有11例、3例子宫内膜样肿瘤中有2例、16例浆液性肿瘤中有9例以及9例透明细胞性肿瘤中有1例呈阳性。它与腺瘤型子宫癌以及子宫颈正常黏膜和输卵管正常表面上皮发生反应。在非妇科肿瘤中，OM - B抗原存在于转移至卵巢的肿瘤以及胃癌和胰腺癌中，而OM - C存在于转移至卵巢的肿瘤以及胃癌和结肠癌中。因此，血清学分析表明这三种单克隆抗体对黏液性和子宫内膜样类型的卵巢肿瘤具有选择性特异性。对这三种OM抗原的初步特性分析表明，它们与癌胚抗原或ABH血型相关抗原不同。
