Department of Pharmaceutical Technology, Jadavpur University, Raja SC Mallick Rd, Kolkata 700032, West Bengal, India. pallab
Environ Toxicol Pharmacol. 2011 Jan;31(1):10-7. doi: 10.1016/j.etap.2010.08.003. Epub 2010 Aug 26.
Present study reports the chemopreventive effect of methanol extract of Wedelia calendulaceae (MEWC) against 20-methylcholanthrene (20-MC) induced carcinogenesis in Swiss albino mice. MEWC was administered orally at 250 and 500 mg/kg body weight for 90 consecutive days after 24h of single subcutaneous administration of 20-MC (200 μg) in mice and observed for 15 weeks to record tumor incidence (fibrosarcoma) and survival. After 15 weeks the mice were sacrificed for the estimation of hematological profiles and liver biochemical parameters viz. lipid peroxidation, reduced glutathione (GSH), glutathione-S-transferase (GST), superoxide dismutase (SOD) and catalase (CAT). MEWC treatment markedly reduced tumor incidence and prolonged life span of sarcoma bearing mice as compared to 20-MC control. Hematological profiles were significantly (p<0.001) restored to normal levels in MEWC treated mice. MEWC treatment significantly (p<0.001) modulated the aforesaid liver biochemical parameters as compared to 20-MC control. Therefore, W. calendulaceae possess remarkable chemopreventive efficacy in Swiss mice.
本研究报告了韦德拉菊属甲醇提取物（MEWC）对 20-甲基胆蒽（20-MC）诱导的瑞士白化病小鼠致癌作用的化学预防作用。在 20-MC（200μg）单次皮下给药后 24 小时，MEWC 以 250 和 500mg/kg 体重口服给药，连续 90 天，并观察 15 周，以记录肿瘤发生率（纤维肉瘤）和存活率。15 周后，处死小鼠以估计血液学参数和肝生化参数，即脂质过氧化、还原型谷胱甘肽（GSH）、谷胱甘肽-S-转移酶（GST）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）。与 20-MC 对照组相比，MEWC 处理显著降低了肿瘤发生率并延长了肉瘤荷瘤小鼠的寿命。与 20-MC 对照组相比，MEWC 处理使血液学参数显著（p<0.001）恢复到正常水平。与 20-MC 对照组相比，MEWC 处理显著调节了上述肝生化参数。因此，韦德拉菊属在瑞士小鼠中具有显著的化学预防功效。
