Determination of antioxidant activity and antineoplastic effects against Ehrlich ascites carcinoma of methanolic extract of (L.) Pruski.

BACKGROUND AND AIM

(L.) Pruski is a well-known medicinal plant that is widely used in traditional medicine. This study was conducted to evaluate the antioxidant and antineoplastic properties of leaf.

EXPERIMENTAL PROCEDURE

Methanol was used as a solvent to prepare leaf extract from the dried leaf of (MESL). We have evaluated the total phenolic and flavonoid contents of MESL, and using five different assays; we have also evaluated the antioxidant property of MESL. antineoplastic activity of MESL against Ehrlich ascites carcinoma (EAC) cells in experimental mice was also explored.

RESULTS

The phytochemical analysis of MESL exhibited the total phenolic, and total flavonoid contents in the dry extracts were 32.86 mg/gm of gallic acid equivalent and 127.26 mg/gm of catechin equivalent, respectively. Moreover, MESL also showed promising scavenging activity in all assays (IC value ≥70.5 μg/mL). In the antineoplastic assay, MESL showed increased EAC cell death at the doses of 50 mg/kg and 100 mg/kg body weight. MESL administration also induced apoptosis of EAC cells, significantly inhibited EAC cell growth, and increased the life span of EAC cell-bearing mice compared to EAC cell-bearing control mice.

CONCLUSIONS

All findings of this study suggest potential antioxidant and antineoplastic properties of MESL.