University of Medical Sciences, Department of Pharmaceutical Biochemistry, Święcickiego 4, 60-781 Poznań, Poland.
Environ Toxicol Pharmacol. 2011 Mar;31(2):339-46. doi: 10.1016/j.etap.2010.12.006. Epub 2011 Jan 18.
Chokeberry is a rich source of procyanidins known to have several types of biological activity including anticarcinogenic potential in experimental models. In this study we examined the effect of chokeberry juice on the hepatic and mammary gland carcinogen metabolizing enzyme expression altered by the polycyclic aromatic hydrocarbon, 7,12-dimethylbenz[a]anthracene (DMBA). Sprague-Dawley rats were gavaged with chokeberry juice (8 ml/kg b.w.) for 28 consecutive days. DMBA was administered i.p. on the 27th and the 28th days. Pretreatment with chokeberry juice reduced the activity of CYP1A1 and increased that of CYP2B involved in metabolic activation/detoxication of DMBA in rat liver, as well as expression and activity of phase II enzymes. Chokeberry juice had no effect on these parameters in the mammary gland and DMBA induced DNA damage in rat blood cells. These results together with our earlier observations indicate that metabolic alterations induced by chokeberry feeding are tissue specific and depend on the class of carcinogen.
沙棘是原花青素的丰富来源，已知原花青素具有多种生物活性，包括在实验模型中具有抗癌潜力。在这项研究中，我们研究了沙棘汁对多环芳烃 7,12-二甲基苯并[a]蒽（DMBA）改变的肝和乳腺致癌物代谢酶表达的影响。Sprague-Dawley 大鼠连续 28 天每天灌胃沙棘汁（8 ml/kg b.w.）。第 27 天和第 28 天腹腔注射 DMBA。沙棘汁预处理降低了 CYP1A1 的活性，增加了 CYP2B 的活性，CYP2B 参与 DMBA 的代谢激活/解毒，以及 II 相酶的表达和活性。沙棘汁对乳腺中的这些参数没有影响，DMBA 诱导了大鼠血细胞中的 DNA 损伤。这些结果与我们之前的观察结果表明，沙棘喂养引起的代谢改变是组织特异性的，并取决于致癌物的类别。
