Krajka-Kuźniak Violetta, Szaefer Hanna, Ignatowicz Ewa, Adamska Teresa, Oszmiański Jan, Baer-Dubowska Wanda
Department of Pharmaceutical Biochemistry, University of Medical Sciences, Poznań, Poland.
J Agric Food Chem. 2009 Jun 10;57(11):5071-7. doi: 10.1021/jf803973y.
Chokeberry is a rich source of polyphenols, which may counteract the action of chemical carcinogens. The aim of this study was to examine the effect of chokeberry juice alone or in combination with N-nitrosodiethylamine (NDEA) on phase I and phase II enzymes and DNA damage in rat liver. The forced feeding with chokeberry juice alone decreased the activities of enzymatic markers of cytochrome P450, CYP1A1 and 1A2. NDEA treatment also decreased the activity of CYP2E1 but enhanced the activity of CYP2B. Pretreatment with chokeberry juice further reduced the activity of these enzymes. Modulation of P450 enzyme activities was accompanied by the changes in the relevant proteins levels. Phase II enzymes were increased in all groups of animals tested. Chokeberry juice augmented DNA damage and aggravated the effect of NDEA. These results indicate that chokeberry may protect against liver damage; however, in combination with chemical carcinogens it might enhance their effect.
黑果腺肋花楸富含多酚，可能会抵消化学致癌物的作用。本研究的目的是研究单独的黑果腺肋花楸汁或与N-亚硝基二乙胺（NDEA）联合使用对大鼠肝脏中I相和II相酶以及DNA损伤的影响。单独强制喂食黑果腺肋花楸汁可降低细胞色素P450、CYP1A1和1A2的酶标志物活性。NDEA处理也降低了CYP2E1的活性，但增强了CYP2B的活性。用黑果腺肋花楸汁预处理进一步降低了这些酶的活性。P450酶活性的调节伴随着相关蛋白质水平的变化。在所有测试的动物组中，II相酶均增加。黑果腺肋花楸汁加剧了DNA损伤并加重了NDEA的作用。这些结果表明，黑果腺肋花楸可能预防肝脏损伤；然而，与化学致癌物联合使用时，它可能会增强它们的作用。
