Trundle D S, Chou P P, Raymond A
Adler Institute of Laboratory Medicine, Morton Plant Hospital, Clearwater, FL 34617.
Clin Chem. 1990 Mar;36(3):554-6.
We evaluated a new microparticle enzyme immunoassay (MEIA) for human choriogonadotropin (hCG) in serum. This hCG assay is fully automated for the Abbott "IMx System," which has a dynamic range extending to 100,000 int. units/L. We tested 321 patients' sera, with hCG values ranging between 0 and 196,000 int. units/L by both the IMx hCG (y) and the Hybritech Tandem-E hCG (x) assays. Results correlated well (r = 0.972, slope = 0.87, y-intercept = 0.7). The IMx hCG assay is sensitive (0.21 int. units/L) and precise (CVs 2.4-8.7% for various hCG concentrations). No carryover to subsequent specimens was observed when specimens with values up to 10(6) int. units/L were tested, nor was any high-dose "hook" effect noted. The IMx hCG assay, which is specific for intact hCG molecules, is rapid (one to six samples in 17 min) and is a valid automated alternative to enzymatic and radioisotopic methodologies.
我们评估了一种用于检测血清中人绒毛膜促性腺激素（hCG）的新型微粒酶免疫测定法（MEIA）。这种hCG测定法针对雅培“IMx系统”实现了完全自动化，其动态范围扩展至100,000国际单位/升。我们采用IMx hCG（y）测定法和海普瑞公司的串联E hCG（x）测定法，对321例患者的血清进行了检测，hCG值范围在0至196,000国际单位/升之间。结果显示相关性良好（r = 0.972，斜率 = 0.87，截距 = 0.7）。IMx hCG测定法灵敏（0.21国际单位/升）且精确（不同hCG浓度下的变异系数为2.4 - 8.7%）。当检测值高达10⁶国际单位/升的标本时，未观察到对后续标本的残留影响，也未发现任何高剂量“钩状”效应。IMx hCG测定法对完整的hCG分子具有特异性，检测速度快（17分钟内可检测1至6个样本），是酶法和放射性同位素法的一种有效的自动化替代方法。
