Ford K A, Baker H N, Baar J G, Balay D, Marlewski D, Venetucci M, Ogunro E A
Department of Physiological Diagnostics, Abbott Diagnostics Division, Abbott Laboratories, Abbott Park, IL 60064.
Clin Chem. 1989 Dec;35(12):2333-5.
An automated enzyme immunoassay for human lutropin for use with the Abbott IMx analyzer is described. The assay provides results in approximately 40 min with a sensitivity of 0.25 int. units of LH per liter for up to 23 serum or plasma samples. Cross-reactivity with follitropin (2000 int. units/L) and thyrotropin (2 int. units/L) was negligible; it was 0.016% with human choriogonadotropin (1 X 10(6) int. units/L). There was no interference by high concentrations of bilirubin (0.5 g/L), hemoglobin (7.50 g/L), or triglycerides (13.5 g/L). Intra-, inter-, and total assay CVs were less than or equal to 3.75%, less than or equal to 7.1%, and less than or equal to 7.94%, respectively. Values obtained with the IMx correlated well (r = 0.98, n = 194) with values obtained with Diagnostic Products' LH Double Antibody RIA, and Serono's LH MAIAclone assay. This assay should be useful for small to medium-size laboratories involved in the clinical diagnosis of reproductive pathology.
本文描述了一种用于雅培IMx分析仪的人促黄体生成素自动酶免疫测定法。该测定法可在约40分钟内得出结果，对多达23份血清或血浆样本的检测灵敏度为每升0.25国际单位促黄体生成素。与促卵泡激素（2000国际单位/升）和促甲状腺激素（2国际单位/升）的交叉反应可忽略不计；与人绒毛膜促性腺激素（1×10⁶国际单位/升）的交叉反应为0.016%。高浓度的胆红素（0.5克/升）、血红蛋白（7.50克/升）或甘油三酯（13.5克/升）均无干扰。测定的批内、批间和总变异系数分别小于或等于3.75%、小于或等于7.1%和小于或等于7.94%。用IMx获得的值与用诊断产品公司的促黄体生成素双抗体放射免疫测定法以及赛诺菲的促黄体生成素MAIAclone测定法获得的值相关性良好（r = 0.98，n = 194）。该测定法对参与生殖病理学临床诊断的中小型实验室应有用处。
