Edwards S, Sands J J
Central Veterinary Laboratory, Weybridge, England.
Dtsch Tierarztl Wochenschr. 1990 Feb;97(2):79-81.
Nineteen monoclonal antibodies (MAbs) with specificity for hog cholera virus (HCV) were prepared. They were used in an immune binding (peroxidase linked) assay to determine the reaction patterns of HCV isolates from Europe, Brazil, USA, Japan and Malaysia, as well as laboratory reference strains of the virus. A further panel of 17 MAbs raised against bovine virus diarrhoea virus (BVDV) was included in the study, together with 5 MAbs raised against a non-HCV pestivirus of porcine origin. All the MAbs were also tested against representative strains of BVDV and border disease virus. Six MAbs were HCV-specific, reacting with all isolates of HCV and none of the ruminant viruses. Among the other HCV MAbs geographical variation in reaction patterns was observed. There was evidence of antigenic distinction between recent European isolates, and archive material originally isolated more than 10 years ago.
制备了19种对猪霍乱病毒（HCV）具有特异性的单克隆抗体（MAb）。它们被用于免疫结合（过氧化物酶连接）试验，以确定来自欧洲、巴西、美国、日本和马来西亚的HCV分离株以及该病毒的实验室参考毒株的反应模式。该研究还包括另外一组针对牛病毒性腹泻病毒（BVDV）产生的17种MAb，以及5种针对猪源非HCV瘟病毒产生的MAb。所有的MAb也针对BVDV和边界病病毒的代表性毒株进行了测试。六种MAb对HCV具有特异性，与所有HCV分离株发生反应，而与反刍动物病毒均无反应。在其他HCV MAb中，观察到反应模式存在地理差异。有证据表明近期欧洲分离株与10多年前最初分离的存档材料之间存在抗原差异。
