Paton D J, Sands J J, Lowings J P, Smith J E, Ibata G, Edwards S
Central Veterinary Laboratory (Weybridge), New Haw, Addlestone, Surrey, UK.
Vet Res. 1995;26(2):92-109.
Sixty-six pestiviruses from ruminant and porcine hosts were analysed with a panel of 76 monoclonal antibodies raised against 9 different viruses. Reactivity was used to construct epitope similarity maps for all of the viruses. Four principal virus subgroups were demonstrated. One subgroup equated to classical swine fever virus (CSFV) and included most porcine pestiviruses but none from ruminants. A second subgroup contained mainly viruses of bovine origin, including reference bovine viral diarrhoea viruses (BVDV) such as NADL; however viruses from pigs and sheep were also represented. A third subgroup represented by reference strains of border disease virus (BDV) comprised mainly ovine isolates, but also viruses from pigs. The fourth and most recently defined subgroup contained no reference strains of CSFV, BVDV or BDV, but included atypical viruses from cattle, sheep and pigs. The subgrouping scheme was supported by genetic comparisons between representative viruses from the 4 subgroups and by virus neutralisation with polyclonal sera.
利用针对9种不同病毒产生的76种单克隆抗体组成的抗体组，对来自反刍动物和猪宿主的66种瘟病毒进行了分析。利用反应性构建了所有病毒的表位相似性图谱。结果显示有四个主要的病毒亚组。一个亚组等同于经典猪瘟病毒（CSFV），包括大多数猪瘟病毒，但没有反刍动物的病毒。第二个亚组主要包含牛源病毒，包括参考牛病毒性腹泻病毒（BVDV），如NADL；不过也有来自猪和羊的病毒。以边界病病毒（BDV）参考毒株为代表的第三个亚组主要由绵羊分离株组成，但也有来自猪的病毒。第四个也是最近确定的亚组不包含CSFV、BVDV或BDV的参考毒株，但包括来自牛、羊和猪的非典型病毒。4个亚组代表性病毒之间的基因比较以及用多克隆血清进行的病毒中和试验支持了这种亚组划分方案。
