Douen A G, Ramlal T, Cartee G D, Klip A
Division of Cell Biology, Hospital for Sick Children, Toronto, Ontario, Canada.
FEBS Lett. 1990 Feb 26;261(2):256-60. doi: 10.1016/0014-5793(90)80566-2.
Insulin and acute exercise (45 min of treadmill run) increased glucose uptake into perfused rat hindlimbs 5-fold and 3.2-fold, respectively. Following exercise, insulin treatment resulted in a further increase in glucose uptake. The subcellular distribution of the muscle glucose transporters GLUT-1 and GLUT-4 was determined in plasma membranes and intracellular membranes. Neither exercise nor exercise----insulin treatment altered the distribution of GLUT-1 transporters in these membrane fractions. In contrast, exercise, insulin and exercise----insulin treatment caused comparable increases in GLUT-4 transporters in the plasma membrane. The results suggest that exercise might limit insulin-induced GLUT-4 recruitment and that following exercise, insulin may alter the intrinsic activity of plasma membrane glucose transporters.
胰岛素和急性运动（在跑步机上跑步45分钟）分别使灌注的大鼠后肢的葡萄糖摄取增加了5倍和3.2倍。运动后，胰岛素治疗导致葡萄糖摄取进一步增加。测定了肌肉葡萄糖转运蛋白GLUT-1和GLUT-4在质膜和内膜中的亚细胞分布。运动和运动-胰岛素治疗均未改变这些膜组分中GLUT-1转运蛋白的分布。相比之下，运动、胰岛素以及运动-胰岛素治疗使质膜中GLUT-4转运蛋白有相当程度的增加。结果表明，运动可能会限制胰岛素诱导的GLUT-4募集，并且运动后，胰岛素可能会改变质膜葡萄糖转运蛋白的内在活性。
