Hirshman M F, Wallberg-Henriksson H, Wardzala L J, Horton E D, Horton E S
Department of Medicine, University of Vermont, College of Medicine, Burlington 05405.
FEBS Lett. 1988 Oct 10;238(2):235-9. doi: 10.1016/0014-5793(88)80486-1.
To determine whether increased glucose transport following exercise is associated with an increased number of glucose transporters in muscle plasma membranes, the D-glucose inhibitable cytochalasin B binding technique was used to measure glucose transporters in red gastrocnemius muscle from exercised (1 h treadmill) or sedentary rats. Immediately following exercise there was a 2-fold increase in cytochalasin B binding sites, measured in purified plasma membranes enriched 30-fold in 5'-nucleotidase activity. This increase in glucose transporters in the plasma membrane may explain in part, the increase in glucose transport rate which persists in skeletal muscle following exercise. Where these transporters originate, remains to be elucidated.
为了确定运动后葡萄糖转运增加是否与肌肉质膜中葡萄糖转运体数量增加有关，采用D-葡萄糖可抑制的细胞松弛素B结合技术，测量运动（跑步机运动1小时）或久坐大鼠的红色腓肠肌中的葡萄糖转运体。运动后立即测量发现，在富含5'-核苷酸酶活性30倍的纯化质膜中，细胞松弛素B结合位点增加了2倍。质膜中葡萄糖转运体的这种增加可能部分解释了运动后骨骼肌中持续存在的葡萄糖转运速率增加的现象。这些转运体的来源尚待阐明。
